Answer the following questions regarding PCR reactions: \\ a. What do dNTPs provide during DNA replication? How are they different from dNMPs?\\ b. Why is Taq DNA polymerase commonly used in PCR, as opposed to, for example, mammalian DNA polymerase?\\ c. ...
Answer the following questions regarding PCR reactions: \\ a. What do dNTPs provide during DNA replication? How are they different from dNMPs?\\ b. Why is Taq DNA polymerase commonly used in PCR, as opposed to, for example, mammalian DNA polymerase?\\ c. ...
(dNTPs, Mg2+, and polymerase). The more targets that are assayed in a multiplex reaction, the more likely it is that there will be competition for reagents and inhibition between assays. Master mixes specifically designed for performing multiplex PCR should be ...
PCR reactions were performed in a total volume of 25 [micro]L, including 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl (pH 9.0), 0.1% Triton X-100, 200 [micro]M dNTPs each (Fermentas, Germany), 25 pmoL of each primer, 1.5 U of Taq DNA polymerase (Fermentas, Germany), and 3 [micr...
By mixing ddNTPs that have been labeled with a different color for each base, unlabeled dNTPs, and template DNA in a polymerase-driven reaction, strands of each possible length are produced when the ddNTPs are randomly incorporated and terminate the chain...
The master mix must be well mixed and then split by the number of reactions, ensuring that each reaction will contain the same amount of enzyme, dNTPs, and primers. Many suppliers, such as Enzo Life Sciences, also offer PCR mixes that already contain everything except primers and the DNA...
Bridge PCR refers to the fact that during the annealing step, the extension product from one primer forms a bridge to the other bound primer and its complementary strand is produced. This sequencing techniques uses reversible terminators bound to dNTPs in a cyclical method that comprises of ...
Empty CellATPOther NTPsadATPOther dNTPsaDNARNA Empty Cell UvrD/Rep family UvrDb + − + − + n.d. 3′–5′ Repc + − + − + n.d. 3′–5′ PcrAd + + + + + n.d. 3′–5′ Pif1-like family Pif1e + + + + + n.d. 5′–3′ RecDf + + n.d. n.d. + n...
What are the steps in DNA replication? How DNA replication occurs? What do dNTPs provide during DNA replication? How are they different from dNMPs? Explain the process of DNA replication. Explain the process of replication. Why does this process produce identical DNA strands?
Why are ddNTPs used in Sanger sequencing, and how do they differ functionally from dNTPs? Do ddNTPs differ from dNTPs functionally since they lack two hydroxyl groups? Does Sanger sequencing minimize t...