RNA-seq, a comprehensive sequencing technique, revolutionizes our understanding of gene expression and transcription regulation within cells. This powerful approach involves a systematic process:Firstly, total RNA, including both mRNA and non-coding RNA, is extracted from cells or tissue sampl...
2. RNA library preparation: the extracted RNA is reverse transcribed, cDNA is synthesized, and adapter sequences are added to it to form an RNA-seq library. 3. Sequencing: RNA-seq libraries are sequenced at high throughput, usually using sequencing platforms such as Illumina or Ion Torrent. ...
What is a template in DNA replication? What is a DNA virus? What is the structure of DNA? What are reads in DNA sequencing? What is the process of DNA sequencing? What is high throughput DNA sequencing? What are the bases that make up DNA?
What is automated DNA sequencing? How is DNA sequencing useful? How does DNA sequencing work? What are reads in DNA sequencing? Why do we do DNA sequencing? What DNA sequencing technique is useful for high throughput sequencing? How does genomics rely on DNA sequencing?
Sequencing of mate-pair libraries generates two reads that are distal to each other and in the opposite orientation. Using the physical information associated between the two sequencing reads, mate pair sequencing is useful for de novo assembly, large stru...
Biological replicates are indispensable in ATAC-seq protocols to ensure reproducibility and reliability. As with RNA-seq, maintaining high-quality standards, particularly in sequencing depth and raw reads processing, is essential. ATAC-seq vs. RNA-seq: A Contrast ...
Poor quantification of lncRNAs due to the low abundance and the lack of completeness in lncRNA annotation For mere detection of the presence of a lncRNA, a few reproducible sequencing reads should suffice. But for quantification, at least hundreds read counts are required to reliably represen...
What are the applications of capillary electrophoresis? NGS Education for Sanger Sequencing Users The use of capillary electrophoresis (CE) in genetic analysis applications has largely replaced the use of gel separation techniques due to significant gains...
In this example you have one sample that is processed through one GEM well then you generate one library which is sequenced across multiple flowcells. This may be done to increase sequencing depth, for example. In this case all of the reads can be combined in a single instance of thecell...
Assay Time2 hours 30 minutes AMPINEXT™ DNA Size Selection Kit ENZ-GEN506 A complete set of optimized reagents for quick removal of DNA fragments of <150 bps for library preparation in next generation sequencing applications. Assay Time30 minutes...