Next-generation sequencing for beginners Curious about using NGS in your research? Learn the basics of next-generation sequencing and find tips for getting started. Learn about NGS Techniques Sequencing DNA Sequencing RNA Sequencing High-Throughput Sequencing Library Preparation Microarrays Multiomics ...
Despite the potential importance of TE-G transcripts in plant developmental traits and environmental responses, limitations of short-read RNA sequencing and a lack of bioinformatic pipelines have hindered the detection and comprehensive analyses of these transcripts in plants. The recent development of lo...
因为长读长测序可以检测DNA和RNA的化学修饰。目前这里有大量不同的DNA和RNA修饰,好多都是没有被检测到和研究的,长读长测序为研究这些修饰的分布和功能打开了新的大门。 Lucas, M.C., Novoa, E.M. Long-read sequencing in the era of epigenomics and epitranscriptomics. Nat Methods 20, 25–29 (2023). ...
Sentieon为完整的纯软件基因变异检测二级分析方案,其分析流程完全忠于BWA、GATK、MuTect2、STAR、Minimap2、Fgbio、picard等金标准的数学模型。在匹配开源流程分析结果的前提下,大幅提升WGS、WES、Panel、UMI、ctDNA、RNA等测序数据的分析效率和检出精度,并匹配目前全部第二代、三代测序平台。 综上所述,本次真相挑战凸...
Sentieon为完整的纯软件基因变异检测二级分析方案,其分析流程完全忠于BWA、GATK、MuTect2、STAR、Minimap2、Fgbio、picard等金标准的数学模型。在匹配开源流程分析结果的前提下,大幅提升WGS、WES、Panel、UMI、ctDNA、RNA等测序数据的分析效率和检出精度,并匹配目前全部第二代、三代测序平台。
RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput short-read sequencing of cDNA. However, as ongoing advances are rapidly yie...
Sequencing accuracy and systematic errors of nanopore direct RNA sequencing Wang Liu-Wei Wiep van der Toorn Max von Kleist BMC Genomics(2024) Identification of a terpene synthase arsenal using long-read sequencing and genome assembly of Aspergillus wentii ...
Learn about the differences between short- and long-read sequencing and when one technology is more preferable than another.
Long-Read Single-Cell RNA Sequencing Reveals the Alternative Splicing Landscape across Mouse Kidney Epithelial Cellsdoi:10.1681/ASN.2024g4cgc40kMegan L. NoonanHaojia WuBenjamin D. HumphreysAmerican Society of NephrologyJournal of the American Society of Nephrology...
Long-read sequencing (LRS) has become increasingly popular due to its strengths inde novoassembly and in resolving complex DNA regions as well as in determining full-length RNA molecules. Two important LRS technologies have been developed during the past few years, includingsingle-molecule, real-ti...