Short-read sequencing, also known as next-generation sequencing (NGS), is a high-throughput sequencing method that enables rapid and cost-effective analysis of DNA or RNA sequences. It involves breaking the DNA or RNA into short fragments and then simultaneously sequencing millions of these fragmen...
(BG). However, previous studies on boar reproduction primarily focused on the testis, with little attention paid to the other glands. Here, we integrated single-molecule long-read sequencing with short-read sequencing to characterize the RNA landscape from five glands of Banna mini-pig inbred ...
These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform. Here we describe four different library preparation protocols for short-read sequencing: cDNA synthesis ...
RNA sequencing (RNA-seq) that utilises short-read next-generation sequencing (NGS) or long-read sequencing (such as PacBio and Oxford Nanopore Technologies sequencing) is a powerful tool in understanding transcriptional diversity and regulation during various biological processes, including hematopoiesis2...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
p pBackground/p pHigh-throughput cDNA synthesis and sequencing of poly(A)-enriched RNA is rapidly emerging as a technology competing to replace microarrays as a quantitative platform for measuring gene expression./p pResults/p pConsequently, we compared full length cDNA sequencing to 2-channel ...
Several categories of small RNAs have been identified; the best characterized are microRNA (miRNA) and small interfering RNA (siRNA). miRNAs are short, non-coding molecules with a typical length of 22 nucleotides. They have been found to play vital roles in post-transcriptional regulation of ...
3’ single-cell RNA-Seq libraries supported and validated on the Onso systemBlog Why SBB could be a game-changer for liquid biopsy research In this article we will investigate how an important new PacBio short-read sequencing chemistry called sequencing by binding (SBB) has the potential to...
Short-read full-length 16S rRNA amplicon sequencing for characterisation of the respiratory bacteriome of captive and free-ranging African elephants (Loxod... Hypervariable region sequencing of the 16S ribosomal RNA (rRNA) gene plays a critical role in microbial ecology by offering insights into bacte...
java -jar RNA-Bloom.jar -long LONG.fastq -t THREADS -outdir OUTDIR Input reads are expected to be in a mix of both forward and reverse orientations. Options-pooland-refare not supported for long-read data at this time. (B) assemble nanopore direct RNA sequencing data: ...