Short-read sequencing, also known as next-generation sequencing (NGS), is a high-throughput sequencing method that enables rapid and cost-effective analysis of DNA or RNA sequences. It involves breaking the DNA or RNA into short fragments and then simultaneously sequencing millions of these fragmen...
These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform. Here we describe four different library preparation protocols for short-read sequencing: cDNA synthesis ...
Learn about the differences between short- and long-read sequencing and when one technology is more preferable than another.
(BG). However, previous studies on boar reproduction primarily focused on the testis, with little attention paid to the other glands. Here, we integrated single-molecule long-read sequencing with short-read sequencing to characterize the RNA landscape from five glands of Banna mini-pig inbred ...
3’ single-cell RNA-Seq libraries supported and validated on the Onso system Blog Why SBB could be a game-changer for liquid biopsy research In this article we will investigate how an important new PacBio short-read sequencing chemistry called sequencing by binding (SBB) has the potential to ...
3’ single-cell RNA-Seq libraries supported and validated on the Onso system Blog Why SBB could be a game-changer for liquid biopsy research In this article we will investigate how an important new PacBio short-read sequencing chemistry called sequencing by binding (SBB) has the potential to ...
Optimizing de novo common wheat transcriptome assembly using short-read RNA-Seq data . BMC Genomics . 2012; 13 : 392 doi: 10.1186/1471-2164-13-392Duan J, Xia C, Zhao G, Jia J, Kong X. Optimizing de novo common wheat transcriptome assembly using short-read RNA-Seq data . BMC Genomics...
RNA sequencing (RNA-seq) that utilises short-read next-generation sequencing (NGS) or long-read sequencing (such as PacBio and Oxford Nanopore Technologies sequencing) is a powerful tool in understanding transcriptional diversity and regulation during various biological processes, including hematopoiesis2...
Figure 1. GeneLab RNA-seq Consensus Pipeline (RCP) (A) The three broad steps of the RCP. The RCP handles (1) data preprocessing to trim sequencing adapters and to provide quality control metrics; (2) data processing to map reads to the reference genome and quantify the number of read cou...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...