How to design a single-cell RNA-sequencing experiment: pitfalls, challenges and perspectives.doi:10.1093/bib/bby007Alessandra Dal MolinBarbara Di CamilloBrief Bioinform
On a recent project, for example, Litterst deployed Sanger sequencing in support of mRNA vaccines production. Typically, such a vaccine is made by first synthesizing the RNA’s corresponding gene fragment, placing it into plasmid forin vitrotranscription, and then purifying the resulting mRNA. Thro...
How do RNA and DNA viruses differ? What is the difference between RNA and mRNA and their sequencing bases? How do DNA and RNA differ? a. DNA is double-stranded while RNA is single-stranded. b. They have different sugars. c. Thymine is present in DNA but not in RNA. d. All statemen...
How do mutations in DNA affect protein synthesis? What DNA sequencing technique is useful for high throughput sequencing? How does DNA (deoxyribonucleic acid) encode information? What is the structure that allows DNA to replicate? How does RNA structure differ from DNA structure?
Of note, this RNA-sequencing methodology identified the expression of 15,200 and 18,463 genes in human islets in the first and second of these studies, respectively (1,2). This has increased, by more than twofold, the number of genes known to be expressed in human b-cells. Importantly,...
enables the interrogation of hundreds to thousands of genes at one time in multiple samples, as well as discovery and analysis of different types of genomic features in a single sequencing run, from single nucleotide variants (SNVs), to copy number and structu...
QN was originally developed for gene expression microarrays13but is now used across almost any kind of high-dimensional/-throughput -omics platform including RNA-sequencing14and proteomics9. A particular danger in the use of QN is that lay analysts are easily misled by the rather “perfect-looking...
Small interfering RNA siRNA Supernova Tagging SunTag Translocation methylcytosine dioxygenase 1 TET1 Ubiquitous chromatin opening elements UCOEs Whole Genome Bisulfite Sequencing WGBS Yin Yang 1 YY1 1. Phenotypic diversity in mammalian production cell lines and its ups and downs Chinese hamster ovary (CHO...
becausedeep-sequencingexperimentsalwaysrequireacutoffofsomeminimumnumberofreads.ItismoredifficulttoconceiveafunctionforalncRNAthatispresentatlessthanonecopypercellthanforlncRNAsthataremoreabundant.VeryrareorunstablelncRNAscouldbetranscriptionalnoise.Furthermore,manyresearchersstudyonecelltype,butlncRNAsareoftenspecificto...
To get a better idea of the role that changes in dendrites play in learning, Hacisuleyman extended the TurboID platform to works in concert with RNA-sequencing, CLIP, translation and protein analysis. The platform allowed the team to track activity in dendrites before, during, and several minute...