[4] Lei H, Fei M, Chapman A, et al. Single-cell whole-genome amplification and sequencing: methodology and applications[J].Annual Review of Genomics & Human Genetics, 2015, 16(1). [5] Wang Y, Waters J, Leung ML, et al. Clonal evolution in breast cancer revealed by single nucleus g...
High Throughput DNA Extraction, WGA and Targeted Next Generation Sequencing (NGS)Kruize, NielsPlant & Animal Genome
Whole transcriptome sequencing of the aging rat brain reveals dynamic RNA changes in the dark matter of the genome nature14878_Whole-genome sequencing identifies EN1 as a determinant of bone density and fracture Whole Genome Sequencing data analysis:全基因组测序数据分析...
DNA sequencing analysis of a 2-kb region within the CFTR gene showed no mutations introduced by WGA. The relatively high uniformity and consistency of the WGA process, coupled with the low replication error rate, suggests that WGA DNA may be suitable for accurate genotyping. Regions of the ...
Investigators found WGA, consisting of whole-genome sequencing and comprehensive variant discovery, revealed infantile epileptic encephalopathy (EIEE) in 14 infants for whom previous genetic tests had not yielded a diagnosis. This strategy, investigators note, supports WGA as a superior diagnostic strategy...
IntroductionThere are several methods to prepare embryo biopsy samples for Next Generation Sequencing (NGS) for Preimplantation Genetic Testing for Aneuploidy (PGT-A). DOPlify? provides a flexible technology to not only amplify whole gen... M Jasper,S Myers - 《Reproductive Biomedicine Online》 被...
polymorphism arrays, SNP),二代测序技术(next-generation sequencing technology, NGS)等。 第四军医大学博士学位论文 -4- WGA 是一种能对微量甚至是极微量DNA 进行有效扩增的技术,其扩增产物可 被多种常规分子技术用于下游的遗传学分析。尽管现有多种被优化的 WGA 技术, 但本质上都可归于扩增前引物延伸反...
errors, dramatically improves time to results and reduces background. ThePicoPLEX WGA Kitcan also be used with isolated gDNA amounts ranging from less than 6 pg to 50 pg. The workflow has been successfully used in a broad range of applications for qPCR, arrays, and...
which after PCR can yield >10 μg of WGA product. After purification, the WGA product can be analyzed in a manner similar to any genomic or chromosomal DNA sample. A number of downstream applications may be performed including TaqMan® assays, microsatellite analysis, SNP analysis, sequencing,...
Uncovering low-level mosaicism in human embryonic stem cells using high throughput single cell shallow sequencing Human pluripotent stem cells (hPSCs) have significant levels of low-grade genetic mosaicism, which commonly used techniques fail to detect in bulk DNA. These copy number variations remain ...