Thousand and one ways to quantify and compare protein abundances in label-free bottom-up proteomics. How to process and analyze MS data to quantify and statistically compare protein abundances in bottom-up proteomics has been an open debate for nearly fift... Blein-Nicolas,M��lisande,Zivy...
One popular metabolic approach for proteomics researchers is SILAC, which stands for stable isotope labeling with amino acids in cell culture. Used to drive mass spectrometry experiments, SILAC enables researchers to quantify differences in protein abundance between two cultures or cell lines by labeling...
chemistry and physiology (Valladares and Niinemets2008). To a certain extent these differences are merely physiological consequences of the environmental conditions: if light levels are low, the photosynthetic rates are necessarily also low. However, plants...
The rising prevalence of obesity around the globe places an increasing burden on the health of populations, on healthcare systems and on overall economies. A major challenge for researchers is to quantify the effect of these burdens to inform public policies. Using a simulation model to project t...
hydrolysates; (ii) stability of growth functions across different strains to establish the impact of perturbations on yeast metabolism; (iii) stability of intracellular parameters over time; (iv) stability of intracellular parameters within a cell population to indirectly quantify...
Cells and supernatant were lysed to extract DNA. Samples were then subjected to absolute real-time quantitative PCR (qPCR) using major capsid protein (MCP) primers to quantify TFV genomic concentration. Statistical analysis was performed using Student’s t-test (0.05 < *p ≤ 0.1, 0...
Immuno-PCR is a technology with no need for dedicated instrumentation, instead using a generic rtPCR thermal cycler to quantify an immobilized oligonucleotide. The immuno-PCR method is similar to an ELISA method wherein the enzyme-linked detection antibody is substituted with an antibody-oligonucleotid...
"Antibodies, or antigen-based diagnostics, are understandably well utilized. Their levels can be used to quantify disease states," Mabbott said. "The problem is it takes a long time for those biomolecules to achieve detectable levels. Levels of disease-related microRNA levels are perturbed much ...
Cells and supernatant were lysed to extract DNA. Samples were then subjected to absolute real-time quantitative PCR (qPCR) using major capsid protein (MCP) primers to quantify TFV genomic concentration. Statistical analysis was performed using Student’s t-test (0.05 < *p ≤ 0.1, 0...