Proteintech experts share ways to quantify your microglial cell population What are microglia? Microglia are the resident immune cells of the central nervous system (CNS) and are key players in brain homeostasis. They account for about 10–15% of the cells found in the brain. In contrast with ...
Simple Western requires only 3 µL of sample and offers highly sensitive multiplex detection to quantify low abundance phosphorylated and total-protein isoforms in a sample. The Stellar™ NIR/IR Modules for the Simple Western instrument Jess™ provide industry-leading fluorescence detection ...
Predicting concentrations is crucial in natural product analysis to quantify specific compounds or groups of compounds in samples. HPLC stands out for its ability to separate, identify, and quantify mixture components. It's employed in determining ephedrine alkaloids in Ephedra products and analyzing Thi...
4C). To directly quantify the rates at which actin, CP, and the Arp2/3 complex join the growing network at the NPF-coated surface, we integrated the fluorescence intensity of each component in individual actin networks and divided these values by the reaction time (Fig. 4D). This analysis...
protein. For such a study to make it into our analysis we require: (i) that the signal be normalized by the activity of the non-denatured protein sample; (ii) that centrifugation be performed before the start of the experiment to remove insoluble aggregates; and (iii) that the fraction ...
The recombinant expression, isolation and characterization of pore-forming proteins is one of the most commonly used strategies for understanding the permeability properties of the biological membrane into which they are embedded. This protocol describes how to quantify the expression of your protein of ...
Genetic analysis technologies including RT-qPCR to quantify viral RNA and expression of experimental host genes, fragment analysis methods to prepare and validate genetic constructs, Sanger sequencing to confirm intended genetic regions and next-generation s...
Because no consensus exists on how to quantify adipose tissue insulin sensitiv... E S?Ndergaard,EDYA Elena,M Morgan-Bathke,... - 《J Clin Endocrinol Metab》 被引量: 14发表: 2017年 1502-P: Adipose Tissue Protein Response and Insulin Sensitivity in Obesity Dysregulation of adipose tissue (...
Ectopic expression of alpha B-crystallin in Chinese hamster ovary cells suggests a nuclear role for this protein. alpha B-crystallin (alpha B) is known to be a cytosolic, small heat shock-like multimeric protein that has anti-aggregation, chaperone-like properties. The... SP Bhat,IL Hale,B...
While we know that a certain level of inflammation is needed to trigger an effective adaptive immune response, we do not yet know how to quantify that level, or predict how this translates into reactogenicity. New technologies including systems biology are providing new insights into the early imm...