Fluorescence screening: Don’t let background noise mask your signals Detecting xFP fusion proteins can be very challenging. You try to keep the expression physiologically low for realistic protein levels. Signals are therefore faint and hard to identify. In this situation your microscope solution sho...
Method overcomes challenges to streamline research workflows and protect thermally sensitive materials. More Bench Tips » Webinars Use of Automated Electrophoresis for Quality Control of Protein Expression and Purification Steps Thursday, April 10, 2025 ...
This format also may be used to present the results of simple protein expression, purification, characterization, and/or application projects using standard approaches. However, the study must be novel in some way, for example if it is the first example of expression and purification of the ...
TheRed Fireflyluciferase is an intracellular protein with an emission peak shifted into the red spectrum compared to the native Firefly luciferase, which allows multiplexing withGaussia,Cypridinaand GreenRenillaluciferases. Spectral emission profile for luc...
TheRed Fireflyluciferase is an intracellular protein with an emission peak shifted into the red spectrum compared to the native Firefly luciferase, which allows multiplexing withGaussia,Cypridinaand GreenRenillaluciferases. Spectral emission profile for ...
Guide to Protein Purification译者: Burgess, Richard R;Deutscher, Murray P. 出版商: Elsevier Science 出版年: 2009 ISBN: 9780080923178 分类: [Q51 蛋白质] 语种: ENG 简介 Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the...
This technique has direct applications in genetic research, enabling scientists to study gene function and protein interactions in detail. The recombinant DNA technology is also crucial in large-scale medicine, where it is used to produce insulin, vaccines, and other therapeutic proteins. Molecular ...
Guide to Protein Purification, Second Edition 463, 191-222 (2009).BURGESS, R.R., MURRAY, P.D.: Guide to protein purification. Elsevier Inc., San Diego, 2009, 851 pp.Jarvis, D.L. Baculovirus-Insect Cell Expression Systems. In Guide to Protein Purification, Second Edition; Burgess, R.R...
We obtained biopsies from three patients at two locations, the nonhealing edge (prior to debridement) and the adjacent, nonulcerated skin of the venous ulcers (post debridement), and evaluated their histology, biological response to wounding (migration) and gene expre...
You must also choose whether a monoclonal or polyclonal is more suited to your needs; monoclonal antibodies give a highly specific signal whereas polyclonal antibodies can give a brighter one, particularly if expression levels of the protein of interest are low. Table 2: Primary antibodies for IHC...