The chromatin accessibility regulates gene expressions60,68. We found that chromatin accessibility around promoter regions was critical for regulating the dynamics of ADEM genes. Both natural aging and LPS challenge induced overt modifications of chromatin structure, leading to microglial reactivity and driv...
To compare escape latency, repeated-measure two-way analysis of variance (ANOVA) with Dunnett's post hoc test was employed. A one-way ANOVA followed by Fisher's LSD test was used to identify statistically significant differences in other behavioral tests and the expressions of proteins. The ...
h The relative protein expressions of PAPP-A and proMBP in placentas from the IUGR-twin and Normal-cotwin in sIUGR. i, j Protein concentrations of PAPP-A (i) and proMBP (j) in umbilical cord blood from the IUGR-twin and Normal-cotwin in sIUGR. Full size image Since the sIUGR ...
The resulting plasmids, termed pMGβ2-6 K (for 6-kbp cDNA) and pMGβ2-8 K (for 7.6-kbp cDNA) contain either the full-length 6- or 7.6-kbp PTPβ2 cDNA between the second intron of rabbit β-globin gene and its poly(A) signal, and their expressions are under the control of ...
Xenobiotic transporters of the ATP-binding cassette (ABC) protein superfamily play important roles in maintaining the biochemical barrier of various tissues, but their precise functions in the skin are not yet known. Screening of the expressions of the known xenobiotic transporter genes in two in vit...
The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively. Results The mRNA and protein levels of YOD1 were higher in ...
As shown in Figure 8: under LFBE treatment, the protein expressions levels of Bcl-2 and cyclinD1 were significantly downregulated compared with the control group (P< 0.05) and the protein expressions levels of Bax and caspase-3 were significantly upregulated (P< 0.05). However, under 5-FU...
To detect the expression of multidrug resistance molecules P-glycoprotein (P-gp), Lung resistnce protein (LRP) and Multidrug resistance-associated protein (MRP) and analyze the relationship between them and the clinico-pathological features. The expressions of P-gp, LRP and MRP in formalin-fixed ...
In the lesions, the mRNA levels of VEGFA and TGFβ1, angiogenic factors, in the DT group significantly decreased to approximately 50% and 30% of control, respectively. Immunohistochemical study revealed the expressions of VEGFA and an endothelial cell marker CD31 in lesions of the DT group, ...
7c, d, g). The expressions of ectodermic marker genes, Pax3 and Fgf5, were increased (Fig. 7e) while the core pluripotency factors, Oct4 and Nanog, were decreased (Fig. 7f). These results indicate O-GlcNAcylation is required for maintenance and self-renewal of mESCs. To study the ...