Included in the kit is an expression control vector (pcDNA3.1/V5-His-TOPO/lacZ©) which you can use to check both transfection efficiencies and expression in your particular cell line. Plasmid Preparation Plasmid DNA for transfection...
Expression While we described protein expression and purification for B. burgdorferi CheAs, CheYs, and CheX, specific measures must be taken to optimize expression and purification for a given protein. For example, a given protein may not express in a given host cell line, but changing the hos...
cell to become tumorogenic and ultimately metastatic. Since proteins are the agents that control these processes, it is important to pinpoint changes in global protein expression in order to identify protein biomarkers involved in these cancer-transforming processes. The ability to identify key prot...
29,30,31,32. Furthermore, several studies have shown that the expression of proteins using cell lysates matches in vivo expression of the same constructs
Expression of Proteins in E. coli Expression of a recombinant protein can be approached in general by constructing a plasmid that encodes the desired protein, introducing the plasmid into the required host cell, growing the host cells and inducing protein expression, and then lysing the cells,purif...
C. A biologically stable DNAzyme that efficiently silences gene expression in cells. Nat. Chem. 13, 319–326 (2021). Article PubMed CAS Google Scholar Wang, Y. Y. et al. An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination. Nat. Chem. 14, 350–...
In this report, we describe the cloning and characterization of a B-type cyclin, Cig2 from the fission yeast Schizosaccharomyces pombe. The cig2 gene encod... T Connolly,D Beach - 《Molecular & Cellular Biology》 被引量: 173发表: 1994年 Genomic expression patterns in cell separation mutants...
Improving protein expression, stability, and function with ProteinMPNN Kiera H. Sumida, Reyes Núñez Franco, Indrek Kalvet, Samuel J. Pellock, Basile I. M. Wicky, Lukas F. Milles, Justas Dauparas, Jue Wang, Yakov Kipnis, Noel Jameson, Alex Kang, Joshmyn De La Cruz, Banumathi Sankar...
5. Ma, H. et al. CRISPR-Cas9 nuclear dynamics and target recognition in living cells.J Cell Biol214, 529-537 (2016). 6. Litke, J.L. & Jaffrey, S.R. Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts.Nat Biotechnol37, 667-675 (2019). ...
2022年11月15日，北京大学生物医学前沿创新中心（BIOPIC）汤富酬团队与北医三院普通外科付卫团队紧密合作，在Protein & Cell上发表了题为Spatially-resolved expression landscape and gene-regulatory network of human gastric corpus epithelium的研究论文。在这项工作中，研究人员首先利用单细胞转录组测序技术构建了人类胃体...