A human hepatocellular carcinoma cell line (HUH-7 Tet-Off) for regulatable protein expression is new and contains a pTet-Off expression vector stably integrated into its genome.doi:DE19815368ERHARDT, ANDREASHAUSSINGER, DIETERNIEDERAU, CLAUSHASSAN, MOHAMED...
Cell Line Development Systems Flp-In™ system—single integration in a random genomic locus, isogenic cell lines. Jump-In™ system—single integration at specific genomic locations called pseudo-attP sites, isogenic cell lines. T-REx™ system—inducible expression of your ...
proteins. This platform is highly advantageous for recombinant protein expression in terms of its strong post-translational modification capability and relatively high cell culture density, which render it an excellent choice for the expression of intracellular proteins, protein complexes, and viral ...
Regulation of protein expression by cyclooxygenase-2 in a human gastric cancer cell line 来自 ResearchGate 喜欢 0 阅读量: 6 作者:NM Griffin,G Cagney,MJ Sullivan,CM Cox,DJ Fitzgerald 摘要: Arterial thrombosis is initiated after atherosclerotic plaque rupture. The ruptured plaque triggers a cascade ...
This confirmed our findings that OCT3/4 protein expression is specific to UNDIF-GCTs and the related cell line models (GCT-CLs). These data are completely in accordance with previously published findings (Looijenga et al, 2003; de Jong et al, 2005). Figure 4 Immunohistochemical detection of...
From the expression pattern in each cell line, we grouped consistent and inconsistent expressions of mRNA and protein. The mRNA and protein expression data were normalized using the mean centered method. This is accomplished by calculating the mean of intensities of each sample, and then scaling ...
The Unfolded Protein Response and Cellular Stress, Part A Gustaafde Ridder, ...Salvatore V.Pizzo, inMethods in Enzymology, 2011 2Methods for Monitoring UPR Modulation 2.1Cancer cell lines The 1-LN cell line (a kind gift from Dr. Phillip Walther, Duke University Medical Center) is a highly...
In order to further investigate the anti-proliferative properties of cell-cycle-targeting miRNAs, we profiled the response of 122 human cancer cell lines from the Cancer Cell Line Encyclopedia (CCLE), representing 12 separate cancer types, to four selected miRNAs. ...
The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.View All Images (2) Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was ...
protein[160]. MCF10A is a non-malignant breast cell line, with few cytogenic abnormalities or mutations [164,165], and hence provides a reasonable model for determining vulnerabilities in cells caused exclusively by the loss ofCDH1expression. In MCF10A cells, the loss ofCDH1causes alterations ...