Here, we quantitatively dissect the maintenance of gene expression in cell lineages for a broad range of cell types. We leverage genes with particularly stable expression over time to develop Gene Expression Memory-based Lineage Inference or GEMLI, a computational tool that allows identifying cell li...
(2005). Gene expression in stem cell-supporting stromal cell lines. Ann. N.Y. Acad. Sci. 1044, 159-167.Charbord P, Moore K. Gene expression in stem cell-supporting stromal cell lines. Ann NY Acad Sci 2005; 1044:159-67.Charbord P, Moore K. Gene expression in stem cell- supporting...
Cell lines are valuable resources as model for human biology and translational medicine. It is thus important to explore the concordance between the expression in various cell lines vis-à-vis human native and disease tissues. In this study, we investigate the expression of all human protein-codin...
Small Cell Neuroendocrine Carcinoma InDiagnostic Pathology: Molecular Oncology, 2016 Gene Expression Profiling • Only a limited number of studies currently describe genome-wide expression profiling in SCNEC ○ Initial studies have shown high-level expression ofINSM1,ASCL1,CHGA,NRXN1, andNCAM1 ...
The spatial and temporal expression during development of a gene(s) that has potential or known importance for developmental mechanisms The temporal expression of large gene sets during an interesting period or process of development Studies of gene expression in cell culture which have clear developmen...
因为前面介绍的都是斑马鱼中的crisper cas9谱系示踪方法,这里再来打卡一篇在小鼠中应用crisper cas9技术的文章,题目是An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells。知乎的标题因为有字数的要求,我这里做了修改,希望不要引起歧义。
Then, density profiles of TFs estimated from the ChIP-seq data are adopted as predictors in a simple linear regression model to predict the genome-wide gene expression. Predictors are also combined with epigenetic data, such as H3K4me3, H3K27me3, DNA methylation, and CpG island [16, 17]. ...
Genetic studies have identified numerous adaptations associated with the shift to agriculture. One of the most well-known examples is the evolution of lactase persistence—the ability to digest lactose, the sugar in milk, into adulthood. In most mammals, the expression of lactase declines after wean...
glia can rapidly respond with changes inprotein expressionoften at places far removed from the cell body. Finally, the actual act of translation of an mRNA into a protein can also be regulated by modulation of proteins that bind the mRNA. In some cases, particularly in development, specific ...
While immortalized cell lines are widely utilized in biological research and provide various advantages including uniformity and accessibility, it is important to confirm that the changes introduced by the immortalization process do not alter the key results. Therefore, top findings from the expression da...