Dna for gene expression and proteins in cellsdoi:US5607843 AThe present invention relates to an isolated nucleic acid fragment comprising a nucleic acid sequence encoding an apamin receptor protein, or biologically active fragment thereof.Patricia T. Sokol...
m6A in mRNA: an ancient mechanism for fine-tuning gene expression. Trends Genet. 33, 380–390 (2017). Article CAS PubMed Google Scholar Vu, L. P. et al. The N 6-methyladenosine (m6A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells. Nat....
Gene expression involves transcription, translation and the turnover of mRNAs and proteins. The degree to which protein abundances scale with mRNA levels and the implications in cases where this dependency breaks down remain an intensely debated topic. H
The cells can be transduced with DNA encoding a therapeutically effective protein such as a blood coagulation factor. The cells are useful in a method for the production of a blood coagulation factor. They can further be used in hemophilia A or B gene therapy....
A plant lectin derived from Viscum album induces cytokine gene expression and protein production in cultures of human peripheral blood mononuclear cells. These results suggest that lectin-sugar interactions on the cell surface of immunocompetent cells can induce cytokine gene expression and protein synthes...
Since sperm cells are transcriptionally and translationally silent, this acquisition of sperm function is highly dependent on posttranslational modifications of proteins (PTMs) [1]. Among the known PTMs, tyrosine phosphorylation events are recognized as the most important hallmark of sperm capacitation....
Proteome analysis is a valuable technique for studying protein expression of organisms in different biological and experimental contexts [3], as well as for finding and annotating novel or unknown genome sequences [4]. Proteins are the final product of gene expression, and essentially the catalytic...
Objective To investigate the effect of c-fos gene silencing on differentially expressed proteins (DEPs) in human bronchial epithelial (HBE) cells after exposure to fine particulate matter (PM2.5). Methods HBE cells and c-fos-silenced HBE cells were exposed to 50 μg/mL PM2.5, LC-MS/MS and...
Moreover, modern approaches are high throughput, allowing characterization of thousands of cells. Nevertheless, genotyping from mRNA remains a challenging and error-prone procedure that, even in the best case, provides incomplete information. For example, stochastic gene expression, biological biases20, ...
MFAP4 multimerises and interacts with elastogenic proteins, including fibrillin-1 and tropoelastin, and with cells via integrins. Structural details of MFAP4 and its potential interfaces for these interactions are unknown. Here, we present a cryo-electron microscopy structure of human MFAP4. In ...