Biochemical test methods for protein quantification can include application of foam-suppressing and/or foam-destroying substances to avoid readout problems and ensure the accuracy of measurement results. One such method for determining the amount of one or more proteins in a solution includes providing...
Total protein quantification methods Protein concentration quantitation is an integral part of any laboratory workflow involving protein extraction, purification, labeling or analysis. Pierce Protein Assays provide a wide range of options for ...
4 Additionally, R&D Systems offers a non-radioactive Universal Kinase Activity Kit that allows for the quantification of kinase activity for any kinase that produces ADP. Although information can be obtained regarding the actions of a specific kinase, assessing enzyme activity in cellular extracts ...
Quantification of absolute labeling efficiency at the single-protein level Super-resolution imaging of reference and target structures enables precise determination of the labeling efficiency of high-affinity binding proteins in cells for improved quantitative assessment of protein organization at the single-...
Understanding protein function and developing molecular therapies require deciphering the cell types in which proteins act as well as the interactions between proteins. However, modeling protein interactions across biological contexts remains challenging for existing algorithms. Here we introduce PINNACLE, a ...
B. Kathrin.New mass spectrometric methods for the quantification of protein-ligand binding in solution, Angew. Chem. Int. Ed . 2004B. Kathrin.New mass spectrometric methods for the quantification of protein-ligand binding in solution, Angew. Chem. Int. Ed . 2004...
Understanding the pros and cons of BCA, Bradford, and UV methods of protein quantificationMeasuring the abundance of proteins in a sample is essential for biological studies and medical diagnostics. For instance, a sudden rise or drop in the concentration of a particular protein after adding ...
This guide provides a comprehensive introduction to DNA and RNA purification methods, including the basics of DNA isolation, plasmid growth and nucleic acid quantification. For ordering information on the products discussed here, please visit our Nucleic Acid Extraction product pages. Go to Products ...
Single-cell proteomics sequencing technology sheds light on protein–protein interactions, posttranslational modifications and proteoform dynamics in the cell. However, the uncertainty estimation for peptide quantification, data missingness, batch effects and high noise hinder the analysis of single-cell prote...
Methods for Quantification of in vivo Changes in Protein Ubiquitination following Proteasome and Deubiquitinase Inhibition Ubiquitination plays a key role in protein degradation and signal transduction. Ubiquitin is a small protein modifier that is adducted to lysine residues b... ND Udeshi,DR Mani,T ...