TRIC: an automated alignment strategy for reproducible protein quantification in targeted proteomicsNext-generation mass spectrometric (MS) techniques such as SWATH-MS have substantially increased the throughput and reproducibility of proteomic analysis, but ensuring consistent quantification of thousands of ...
The advent of fluorescence nanoscopy techniques like Stimulated Emission Depletion microscopy (STED)1,2, Stochastic Optical Reconstruction Microscopy (STORM)3and Photoactivatable Localization Microscopy (PALM)4, meant a revolution in the field of fluorescence-based imaging. Conceptually, they provide a spati...
Quantification of the transport protein distribution was significantly improved by the introduction of the zone model. Earlier, only statistical variances of protein intensity profiles were used [6,7,10,11,13]. Subdivision of the intensity profiles into biologically meaningful zones improved the quantitat...
Last, our program allows spindle detection in other nematode species, offering the same quality of DIC images but for which techniques of transgenesis are not accessible. Thus, our program also opens the way towards a quantitative evolutionary approach of spindle dynamics. Overall, our computer ...
In addition to the emerging non-invasive molecular and anatomical imaging techniques to visualize and quantify deficits and recovery in these disorders, behavioral testing is frequently the primary experimental readout to assess therapeutic effects. For this reason, sensitive, reproducible, time-efficient ...
Microfluidics-based platforms such as LabChip GXII (PerkinElmer) have been applied to the high-throughput analysis of protein size and purity [29,30,47]. However, chip-based techniques use denaturing conditions and lack sufficient resolution for the separation of aggregates which constitutes a bottl...
Design.-During shortages, we implemented an automated algorithm that triggered sending urine samples to the automation line for quantification of protein and ... J Guarner,GH Smith,Alter, David N.Williams, Cecellitha J.Cole, LisaCampbell, Davette G.Elsea, Suzanne M.Reynolds, StacianLawrence, ...
Buoyant density separates EV from lipoprotein particles and protein aggregates in blood; and from Tamm-Horsfall protein (THP) complexes and soluble proteins in urine [9, 10, 20]. In general, a combination of techniques is necessary to first concentrate EV and then increase specificity by ...
(withpvalue threshold for rank invariance of 0.2) was used for normalization38. Protein quantification was performed using R-rollup, which scales the peptides associated with each protein by a reference peptide and then sets their median as the protein abundance39. The peptide having the least ...
In most studies, counting of the microglomeruli was performed manually in small regions of interest (ROIs) after immunostaining synapsin, a protein located in the axon terminals of neurons8. Manual counting is an adequate method to study relative changes in the microglomerular density. However, it...