A. (2006) Automated identification and quantification of protein phosphorylation sites by LC/MS on a hybrid triple quadrupole linear ion trap mass spectrometer. Mol. Cell. Proteomics 5, 337-346Williamson, B.L.,
We incorporate and introduce a multitude of both standard and advanced quantification techniques to extract a hierarchical pool of descriptive multilevel spatial and temporal features to choose from. The whole pipeline can be run on a CPU or accelerated on a GPU, either of which computationally ...
Despite the inherent bias of peptide sampling in single-CV FAIMS compared with multiple CVs and without using FAIMS, we show that the overall protein quantification of optimized single CV (−35 V) correlates strongly with not using FAIMS, which generally is recognized as the “ground truth.”...
(withpvalue threshold for rank invariance of 0.2) was used for normalization38. Protein quantification was performed using R-rollup, which scales the peptides associated with each protein by a reference peptide and then sets their median as the protein abundance39. The peptide having the least ...
this example, are unique peptide sequences of β-CN, and are not found in the other four proteins. The process was repeated for all five milk proteins and a list of signature marker peptides was shortlisted....
In contrast to other modularization techniques [32–35] where the modularity is deduced from the entire model, layers can be modeled independently from each other once their connections are defined. This may give the impression that the user defines the modularity, however, the modularity is unique...
Mass spectrometry (MS) assays also allow for targeted multi plexed protein quantification in complex clinical samples including a variety of tissue matrices (fresh, frozen, formalin-fixed, paraffin- embedded). This can be leveraged for a variety of target engagement assays, the nature of which...
Here, using WSA on a large series of cases with uniform pathology, surgical procedure, standardised tissue processing, staining and selection of anatomical compartment, thus forming an optimal group of cases to address this research question, we found that all techniques showed a significant increase...
In addition to the emerging non-invasive molecular and anatomical imaging techniques to visualize and quantify deficits and recovery in these disorders, behavioral testing is frequently the primary experimental readout to assess therapeutic effects. For this reason, sensitive, reproducible, time-efficient ...
Signal generating chemistries for chemiluminescent techniques include one of either of two formats, each of which cause emission of light from the surface of the processed analytical elements (beads) to produce varying light intensities in response to the concentration of a sample analyte to be ...