The protocols is suitable for analysis of soluble and insoluble protein species (SDS solubilization). Efficient detergent removal and subsequent digestion was automated on a KingFisher Duo using MagReSyn Carboxyl magnetic microparticles. We demonstrated that up to 12 samples could be processed in ...
(0.49 g, 0.53 mL, 3.73 mmol) and AIBN (22.3 mg, 0.2 mmol). (For the synthesis of the NIPs, the template was omitted from the synthetic protocols). The four solutions were then ultrasonicated for 10 minutes at ambient temperature and purged with oxygen-free nitrogen ...
These samples were obtained from the NHS Lothian NRS BioResource Research Tissue Bank (Ethical status/approval ref: 10/S1402/33), conforming to protocols approved by East of Scotland Research Ethics Service (REC). Clinicopathological, treatment and survival data (see Table 1) was collected for ...
QIAGEN 37900 Qproteome Bacterial Protein Prep Kit QIAGEN 9015337 Regulator, DC5V QIAGEN 9015323 O-rings, plunger fixing, M48 (6) QIAGEN 133035 NeXtalStock Ammonium phosphate (200) QIAGEN 133033 NeXtalStock Cobalt(II) Chloride (200) QIAGEN 9001637 Rotor-Gene Q 2plex HRM (w/o PC) System ...
QIAGEN 37900 Qproteome Bacterial Protein Prep KitQIAGEN 9015337 Regulator, DC5VQIAGEN 9015323 O-rings, plunger fixing, M48 (6)QIAGEN 133035 NeXtalStock Ammonium phosphate (200)QIAGEN 133033 NeXtalStock Cobalt(II) Chloride (200)QIAGEN 9001637 Rotor-Gene Q 2plex HRM (w/o PC) SystemQIAGEN 239045 ...
Immunocytochemistry was performed according to standard protocols. Cells were seeded on poly-L-lysine (PLL, 0.1 mg/mL, Sigma-Aldrich)-coated 13 mm glass coverslips (VWR) in a 4-well dish (VWR). The next day, the medium was replaced with starvation medium (0.5% FCS) to induce ciliogenesis...
Western blotting is a very reliable method to measure protein quantity and provide protein identification using antibodies and molecular weight (MW). Other methods for protein quantification, such as Bradford assays and UV-Vis spectroscopy, are not as specific or sensitive as Western blotting. They ...
Thus, efforts to automate liquid transfer steps of cell lysis, protein extraction, and digestion are attractive to both minimize variation and improve resource utilization. While several automated sample preparation protocols have been developed previously, they utilize detergents or chaotropes that must ...
Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores. Nat. Commun. 9, 1–8 (2018). CAS Google Scholar Rodriguez-Larrea, D. & Bayley, H. Multistep protein unfolding during nanopore translocation. Nat. Nanotechnol. 8, 288–295 (2013). CAS PubMed Pub...
et al. Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates. Sci. Transl Med. 6, 219ra9 (2014). Article CAS Google Scholar Agasti, S. S., Liong, M., Peterson, V. M., Lee, H. & Weissleder, R. Photocleavable DNA barcode–antibody conjugates ...