High-throughput characterization of protein–protein interactions by reprogramming yeast mating. Proc. Natl Acad. Sci. USA 114, 12166–12171 (2017). CAS PubMed Google Scholar Browning, D. F. & Busby, S. J. The regulation of bacterial transcription initiation. Nat. Rev. Microbiol. 2, 57 (...
Automated identification and quantification of protein phosphorylation sites by LC/MS on a hybrid triple quadrupole linear ion trap mass spectrometer. Mol Cell Proteomics. 2006; 5 :337–346.Williamson B. L., Marchese J., Morrice N. A. Automated identification and quantification of protein ...
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QIAGEN 37900 Qproteome Bacterial Protein Prep Kit QIAGEN 9015337 Regulator, DC5V QIAGEN 9015323 O-rings, plunger fixing, M48 (6) QIAGEN 133035 NeXtalStock Ammonium phosphate (200) QIAGEN 133033 NeXtalStock Cobalt(II) Chloride (200) QIAGEN 9001637 Rotor-Gene Q 2plex HRM (w/o PC) System ...
Photocleavable DNA barcode–antibody conjugates allow sensitive and multiplexed protein analysis in single cells. J. Am. Chem. Soc. 134, 18499–18502 (2012). Article CAS Google Scholar Kishi, J. Y. et al. SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and ...
Overall, the values were excellent for most analytes, permitting quantification in the pg/ml range of peptide concentration, corresponding to the ng/ml range of protein concentrations (assuming complete trypsin digestion). Comparison of these levels with the performance characteristics reported in Table ...
Theflag wasn't included in the commandTheflag specifies a GPU device to use for accelerate basecalling. If not included in the command, GPU will not be used. GPUs are counted from zero. An example is, when 2 GPUs are specified to use by the Guppy command. ...
We automate the discovery of O-mRNAs that lead to up to 40 times more protein, and are up to 50-fold more orthogonal, than previous O-mRNAs; protein yields from our O-mRNAs match or exceed those from wild-type mRNAs. These advances enable a 33-fold increase in yield for incorporating ...
(Illumina, Cat. No. 20028319)14. The 19.5-h rWGS methods were library preparation from EDTA blood samples with Nextera DNA Flex Library Prep kits (Illumina, Cat. No. 20018705) and five cycles of PCR, 2 × 101 nt sequencing without indexing on NovaSeq 6000 instruments with S1 flow...
The system will analyze samples of serum, plasma, or urine, and specific chemistry kits may also handle clarified cerebrospinal fluid or saliva. The manner of handling certain conceivable errors in the practice of the assay on the inventive instrument is as follows. If there is a lack of ...