Protein preconcentration is an essential sample preparation step for analysis in which the targeted proteins exist in low concentrations, such as bodily fluids, water, or wastewater. Nonetheless, very few practical implementations of miniaturized protein preconcentration devices have been demonstrated in prac...
MSstats is an R-based/Bioconductor package for statistical relative quantification of peptides and proteins in mass spectrometry-based proteomic experiments. It is applicable to multiple types of sample preparation, including label-free workflows, workflows that use stable isotope labeled reference proteins...
Quantification studies on N2 fixation by legumes in the tropics and subtropics have revealed a large range in estimates of the % Ndfa, e.g, 0–95% for soybean, 8–89% for cowpea, 22–92% for groundnut, and 10–88% for pigeonpea [19]. The amounts of N fixed by these legumes range...
Label-based quantification using TMT and iTRAQ. Multi-level detailed reports for peptides, ions, and proteins. Support for REPRINT and MSstats. How to Use Philosopher is part ofFragPipewhich has a user-friendly GUI. Documentation See thedocumentationfor more details about the available commands. ...
The use of a microfluidic "lab-on-a-chip" technique for the separation and quantification of milk proteins is described and compared with traditional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The microfluidic chip technique could separate all major milk proteins when standar...
reported a LOC device integrated with microvalves and a microelectrode for the continuous monitoring of hepatocyte-secreted proteins in the presence of toxic drug acetaminophen (APAP) [93]. APAP is usually used to validate the newly developed LOC systems due to its known hepatotoxicity. In this ...
The dried leguminous seeds contain carbohydrates, proteins, and lipids easily consumed by bruchids. The adult lays eggs on the pod or seed surface, which hatch into larvae and burrow through the pods into the seed, feeding on the nutrient-rich cotyledon. The larvae grow utilizing the food ...
Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics f
A counter provides accurate quantification results. Depending on the features in the automated counter, they can provide information on the viability of cells, handle larger sample sizes, report the concentration, and provide information on the size or even type. Here, we’ll look at the ...
Peptides shared by multiple genes or multiple protein isoforms of the same gene complicate protein inference and quantification. Based on Occam’s razor or the principle of parsimony, the current best practice in the proteomics field is to collapse proteins with the same or subset of supporting pe...