Protein preconcentration is an essential sample preparation step for analysis in which the targeted proteins exist in low concentrations, such as bodily fluids, water, or wastewater. Nonetheless, very few practical implementations of miniaturized protein preconcentration devices have been demonstrated in prac...
This makes the detection and quantification of nucleic acids (260 nm) and proteins (280 nm) possible. BMG LABTECH absorbance microplate readers are equipped with a xenon flashlamp for absorbance measurements and hence provide maximum flexibility to measure any wavelength between 220 and 1000 nm, ...
MiRs are secreted in blood plasma and thus may report on systemic aberrations at an early stage via liquid biopsy analysis. We present a method for multiplexed single-molecule detection and quantification of a selected panel of miRs. The proposed assay does not depend on sequencing, requires less...
Protein quantification assays Bradford, Lowry, ELISAs, BCA Cell viability assays MTT, WST, XTT Microbial growth assay OD600 Fluorescence intensity (incl. FRET) Fluorescence is the absorption of light energy and its transformation into emission light, next to kinetic energy and heat. Since the emitte...
Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics f
The DNA in our bodies is wrapped around proteins called histone. The histone and DNA are covered in chemical tags. This structure is called an epigenome. The epigenome shapes the structure of the genome. Epigenetic marks are modifications of DNA and histones. The epigenome tightly wraps inactive...
Label-based quantification using TMT and iTRAQ. Multi-level detailed reports for peptides, ions, and proteins. Support for REPRINT and MSstats. How to Use Philosopher is part ofFragPipewhich has a user-friendly GUI. See thedocumentationfor more details about the available commands. ...
Garner DL, Gledhill BL, Pinkel D, Lake S, Stephenson D, Van Dilla MA, Johnson LA. Quantification of the X- and Y-chromosome-bearing spermatozoa of domestic animals by flow cytometry. Biol Reprod. 1983;28(2):312–21. CASPubMedGoogle Scholar ...
The protein research being carried out to treat COVID-19 patients is also favorable for the market growth. For instance, in June 2022, scientists at the Indian Institute of Science, Bangalore, developed a unique class of synthetic peptides or miniproteins, and the researchers claim that it had...
“Development of measures for quantification of µs-PEF effects on microtubules”), with the results presented in Fig.4e. As shown in the graph, the value of the MT displacement index speed was proportional to the applied pulse voltage. This finding is understandable, because the electric ...