(2000) Antibody and virus: binding and neutralization . Virology 270 : 1–3Burton D R,Williamson R A,Parren P W. Antibody and virus: Binding and neutralization [J]. Virology,2000,270: 1-3.Burton DR, Williamson RA, Parren PW. Antibody and virus: binding and neutralization. Virology. ...
Antibody responses following HCMV infection of huBLT mice were assessed by enzyme-linked immunosorbent assay (ELISA). Costar clear polystyrene high-protein-binding enzyme immunoassay (EIA) plates (Corning) were coated in either purified HCMV virion preparations (5 ug/mL) diluted in PBS or recomb...
The sample antigen competes with a reference antigen for binding to a specific labelled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is pre-incubated with labelled antibody and added to the wells. Depending on the amount of antigen in the sample, the free ...
The binding of the IgG and the IgM isotypes of human mAb 2F5 to clades A through AE were also evaluated. The binding of 2F5 to HIV-1 was dependent upon the antibody isotype. Monoclonal IgM antibodies bound significantly lower amounts of HIV-1 than the corresponding IgG isotype. Although ...
The nonparametric Wilcoxon’s test for paired samples was used to compare adjacent time points for VZV DNA, antibody titers, and ex vivo ICS. The Wilcoxon’s test was also used to compare T cell breadth or phenotype metrics between HZ and CON skin samples. Pairwise testing was performed due...
Screening testsfor HIV measure the presence of specific antibody in the patient’s serum, detectable after the host has been exposed to the virus for some time (window period). Enzyme immunoassays (EIAs) have developed over time from first to third generation assays. With each new generation th...
although a minority may have detectable anti-HBc IgM for up to 2 years. The anti-HBcantibody titerslowly declines but remains detectable for many years after self-limited infection. The anti-HBc titer usually remains high during the course of chronic infection. During recovery from an acute infe...
The adaptive immune response to influenza virus infection is multifaceted and complex, involving antibody and cellular responses at both systemic and mucosal levels. Immune responses to natural infection with influenza virus in humans are relatively broad and long-lived, but influenza viruses can escape...
ELISA, which involves binding the ligands to surface-immobilized antibodies before adding a large excess of a second antibody which binds a different epitope of the surface-bound ligands, can achieve detection limits between 1 pmol mL−1and 0.1 amol mL−1depending on the antibody-an...
et al. Altered specificity of single-chain antibody fragments bound to pandemic H1N1-2009 influenza virus after conversion of the phage-bound to the soluble form. BMC Res Notes 5, 483 (2012). https://doi.org/10.1186/1756-0500-5-483 Download citation Received21 February 2012 Accepted31 August...