Sarmiento RE, Tirado RG, Valverde LE, Gómez-García B. Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line. Virol J. 2007; 4 :68. doi: 10.1186/1743-422X-4-68. [ Cross Ref ]...
Anti-H5N1 antibody was determined by microneutralization, hemagglutination inhibition, and Western blotting assays in serial blood samples collected from eight Thai patients, including four fatal cases and four survivors. The antibody was detected as early as 5 days and, typically, with an increase in...
The proximity of the beads to the targets in the solution translates directly to short incubation times and therefore fast protocols. Here we take advantage of the negative charge of vesicles combined with the rapid binding kinetics of the Dynabeads™ magnetic beads. Positively charged...
et al. Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection. J. Virol. 82, 115–125 (2008) Article CAS PubMed Google Scholar Montefiori, D. C. Evaluating ...
Longitudinal proteome-wide antibody profiling in Marburg virus survivors identifies wing domain immunogen for vaccine design Surender Khurana Gabrielle Grubbs Andrew S. Herbert Nature Communications(2024) Variant-proof vaccines — invest now for the next pandemic ...
Kinetics of Antibody Binding Using Surface Plasmon Resonance All experiments were performed on a Biacore X100 (GE Healthcare) instrument at 25°C. HBS-EP (10 mM HEPES, pH 7.4, 150 mM sodium chloride, 3 mM EDTA, 0.05% Polysorbate 20) was used as the running buffer and data were analyzed...
Rabbit IgG (Sigma, St Louis, MO) was used as isotype control for detection of non-specific binding. Secondary antibody labeled with Alexaflour-488 was purchased from Life Technologies. Briefly, 5×106 cells infected with influenza A were washed in 2 ml PBS, pelleted by centrifugation, ...
Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin. PNAS. 2011;108(34):14216–21. Article CAS PubMed PubMed Central Google Scholar Mozdzanowska K, Maiese K, Furchner M, Gerhard W. Treatment of influenza virus-infected SCID mice with...
Atomic modelling of this interface is supported by mutagenesis and anti-VEEV antibody binding competition assays. Notably, VEEV engages LDLRAD3 in a manner that is similar to the way that arthritogenic alphaviruses bind to the structurally unrelated MXRA8 receptor, but with a much smaller ...
Biomolecular interactions, such as antibody-antigen binding, are fundamental to many biological processes. At present, most techniques for analyzing these interactions require immobilizing one or both of the interacting molecules on an assay plate or a sensor surface. This is convenient experimentally but...