PROBLEM TO BE SOLVED: To provide a novel antibody binding to influenza virus.SOLUTION: An antibody consists of an amino acid sequence represented by the following structural domain in the direction from N to C: N-FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4-C, wherein CDR1 consists of an amino ...
"The two have high similarity in their receptor-binding domain (RBD)," Zhong said, noting that research show CR3022, a SARS-CoV-specific human monoclonal antibody, could bind potently with 2019-nCoV RBD thus neutralize the coronavirus infection. A medical worker treats a patient at an isolation...
(Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access, and then diluted into binding buffer (50 mM Tris pH 8.0, 150 mM NaCl, 2 mM EDTA, and 1% NP-40) to make a mixed ...
(刺突蛋白RBD负责触发这种病毒与宿主细胞的融合,从而实现对后者的接管)。然而,来自康复患者的一些抗体通过与病毒刺突蛋白上的另一个地方——N末端结构域(N-terminal domain, NTD)——结合来阻断这种病毒。在 MERS 冠状病毒中,靶向 NTD 表位的中和单克隆抗体7D10通过空间位阻阻止RBD 与其细胞受体 DPP4 结合。有趣的...
(HAI) assay, which measures the ability of antibody to inhibit virus–receptor interaction. Several less broad bnAbs recognize the receptor-binding site (RBS) in the HA head, hence exhibiting HAI activity7. Since most universal influenza vaccine candidates aim to induce protective levels of such ...
1. AuthorPradeep D Uchil目前在耶鲁大学病原微生物系工作。Pradeep从事病毒学、免疫学和细胞生物学的研究。他们目前的项目是“TRIM蛋白和逆转录病毒免疫学对先天免疫的调节”。随着新冠的爆发,Pradeep团队也进行…
The rJEV-DIII generated high IgG antibody and its isotypes (IgG2a and IgG3) and induced significant expression of INF-γ and IL-2 cytokines. The rJEV-DIII induced significant lymphoproliferation of splenocytes. In conclusion rJEV-DIII induced Th1 type of immune response which plays an ...
For immunohistochemistry, antibody 3238 to TFIIIC-102 [29] was used at a dilution of 1:100 following standard techniques. Immunofluorescence was carried out on Ad/AH-Neo and -EBNA1 cells using standard procedures [35] with total ATF-2 antibody (mouse MAB1536, 0.5 mg/ml, Santa Cruz). ...
Secondary anti-HRP rabbit antibody (1:5000, NA9340V, GE healthcare), Secondary anti-HRP mouse antibody (1:2000, NA931V, GE healthcare) and goat anti-rat IgG (H&L) HRP antibody (1:5000, 31470, ThermoFisher Scientific). Rabbit Trueblot anti-Rabbit IgG HRP (1:1000, Rockland 18-8816-...
structure elements and the TuYV sequence.cSuperposition of TuYVNRTD with tomato bushy stunt virus (TBSV) coat protein (PDB:2TBV; sequence identity: 9% (across the P domain); DALI26Zscore: 7.0; RMSD: 2.7 Å; P domain, light green; S domain, teal).dTopology of TBSV P domain. ...