该论文综述了DNA和RNA病毒在植物体内诱导VIGS的分子机制,以及VIGS作为基因调控工具在基因功能分析中的各种应用。 由于没了底稿,想了想还是拖一拖,后面有机会再写上面规划的目录。今天就轻松些,直接上网址。 SGN VIGS Tool:https://vigs.solgenomics.net/ 使用简单,把自己需要沉默的基因cds序列粘贴进去直接RUN即可。SG...
Stand Alone version of SGN VIGS Tool Developed on a Catalyst framework To add a VIGS dataset, use the following commands: unzip GCA_003287315.1.zip in emacs, replace the string >lcl| with > , if present (otherwise, fastacmd will not work correctly) Copy the dataset to the correct locatio...
2015. The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics. Molecular Plant 8: 486-488.Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS tool: user- friendly software to design virus-induced gene silenci...
TheSGNVIGStoolalgorithmsimulates in silico the VIGS pro- cesses occurring in the plant cell (Figure 1A). In the first stage, the target gene is processed in all possible 21-nucleotide frag- ments (or any size chosen by the user between 18 and 24 nucle- otides), to get short sequenc...
To install the tool independently of the SGN, a stand-alone version was developed that can be installed as a web tool running on a Catalyst server (https://github.com/solgenomics/VIGS). Not all plant species are amenable to VIGS, and different species show different silencing efficiencies. ...
target: /home/VIGS container_name: vigs_tool image: breedbase/vigs_tool:v0.01 restart: always 开启docker docker compose up-d#输出[+]Running1/1✔Containervigs_tool Started 添加基因组文件 进入docker,想办法把cds文件扔里面,比如windows打开扔进刚才映射的文件夹$HOME/vigs_sequence_files里 ...
Virus-induced gene silencing (VIGS) is a transient assay system widely used in plants to decipher the function of target gene(s). VIGS is a versatile tool that has proven its potential in many plant families. It was first used for dicots, but now many VIGS vectors are also available for...
Thus we introduceldquovirtual grid simulatorrdquo (ViGs), developed as a large scale grid environment simulator, with the goal of studying the performance, behavioral, and scalability aspects of a working grid environment, while catering to the needs for an underlying networking infrastructure. 展开 ...
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A. The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics. Mol. Plant 8, 486-488 (2015).Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing...