The expression profile ofviperintranscripts in EPC-EC cells in response to recombinant type I IFN and to viral infection was determined by RT-qPCR. For this purpose, two different viruses were used: viral hemorrhagic septicemia virus (VHSV), an enveloped negative-sense single-stranded RNA virus b...
The expression of selected metabolic genes was also assessed using qPCR. Unexpectedly, 3 month-old fed or fasted, or 7 month-old fed VLCADmouse hearts displayed values for exogenous long chain FAO that were similar to controls, suggesting a compensatory mechanism, although the partitioning of ...
Such concentration is considered predictive, as SARS-CoV-2 particles have been identified and quantified via RT-qPCR assays applied for SARS-CoV-2 RNA detection. Therefore, the units of measurement are not comparable or convertible into “μg/L”. The control group consisted of fish kept in ...
None of the RNAi clones affected the increased susceptibility of the head muscles to polyQ aggregation (measured as a ratio of head to body aggregation) in either background. This may potentially indicate that more than one gene in the interval was responsible for the switch in the head muscle...
In addition to the two copies of pmoCAB1, many Methylocystaceae members were shown to possess a third pMMO-encoding gene cluster, termed pmoCAB2 (Yimga et al. 2003). Enzymes encoded by pmoCAB1 (pMMO1) and pmoCAB2 (pMMO2) differ in their affinity for CH4 (Figure...
products using the above method, but for which microsatellites could be scored (below), were subjected to a TaqMan qPCR (Applied Biosystems) assay [73] in order to definitively confirm or assignGp-9genotype. Multilocus genotypes at 14 microsatellite loci (Additional file4: Table S2) were ...
293 T cells were transfected with wt and mut plasmids. B,C, GFP-F13A1 mRNA and protein expression were quantified via qPCR and western blot ***p < 0.01. Full size image Effects of the suppression of NMD by CHX To assess whether the decrease in the abundance of mRNA levels in ...
Lung and brain tissues were isolated and Kcna1 gene and protein expression were evaluated by reverse transcriptase quantitative polymerase chain reaction (RT ‐ qPCR) and Western blot techniques. Airway smooth muscle responsiveness was assessed in isolated trachea exposed to MC h. Results Kcna1 ?/?
For the analysis of gene expression, synthetic outer membrane protein A (OmpA) mRNA was added to all samples prior to RNA extraction and used for normalization as described [25]. The RNA levels were analyzed by RT-qPCR for the following genes: tubulin, heat shock protein 70 (HSP70), heat...