The aposymbiotic status of the newly emerged beetles was checked using the culture dependent technique and the qPCR analyses targeting the 16S rRNA genes, respectively. Ten normal and ten antibiotics treated beetles, were randomly selected and individually dissected. Individual guts were removed and hom...
Transcription is the gene expression process responsible for producing all RNA and is a common engineering target for creating novel products, including microbial chemical factories, toxin-sensing genetic circuits, and mRNA vaccines1,2,3. However, while DNA assembly techniques enable the construction of...
Isolated or detected throughout the whole digestive tract, LAB only represent a minor proportion of gastro-intestinal microbial communities [73,91]. Typically, representatives of theLactobacillus/Enterococcusgroup constitute 0.01-1.8% of the overall fecal microbiota, as shown by qPCR techniques [92]. ...
DNA-SIP coupled with shotgun metagenomic sequencing was performed to identify the phylogenetical affiliation of SbOB and investigate their key metabolic functions involved in Sb(III) oxidation. In addition, qPCR and RT-qPCR were performed to identify the role of aioA genes in Sb(III) oxidation....
Nrf2, NF-κB, IL-1β, and NLRP3 mRNA expressions were measured from peripheral blood mononuclear cells (PBMC) using quantitative real-time polymerase chain reaction (qPCR).There were significant positive correlations between TMAO and plasma levels of pCS, NLPR3 inflammasome mRNA expression, serum ...
DNA extraction from tumour samples and qPCR assays for detectingpks+E. coli, ETBF and F. nucleatum The tumour rich regions of the FFPE CRC tumour tissue were macrodissected as previously described for the ACCFR and MCCS studies [28,43]. For the ANGELS study, the genomic DNA was extracted ...
using long-read sequencing or qPCR to measure resistance gene copy number23,24,25,26,27,28,29,30,31,32,33. However, it is not known whether these cases represent a broader trend. To address this question, we examined the distribution of duplicated genes in tens of thousands of complete ...
2–4). The PCR strips were then removed, vortexed, and spun down, and the green fluorescence of each of the samples was measured using the Strategene MX3005p qPCR (Agilent) and an unamplified FAM filter. To measure cell density, the samples were diluted 1:4 with fresh LB media (without...
RNA extraction, cDNA synthesis and RT-qPCR Total RNA was extracted from leaves using trizol reagent (Invitrogen). DNA contamination was removed using RNase-free DNase (TURBO DNAfree kit, Ambion, USA). RNA was quantified using a Colibro microvolume spectrophotometer (Titertek, Brethhold). cDNA ...
(SIGMA, St. Louis)46and are reported in Table1. Relative gene expression was quantified using qPCR with FAST SYBR green reporter (Cat# 4385614) (ThermoFisher, Waltham). β actin (ACTB) mRNA abundance was used as a housekeeping gene for the purpose of normalization. Results are reported as ...