Taylor SC, Laperriere G, Germain H. Droplet digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Sci Rep. 2017;7(1):2409. doi:10.1038/s41598-017-02217-x DeCoste NJ, Gadkar VJ, Filion M. Relative and absolute ...
Taylor SC, Laperriere G, Germain H. Droplet digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Sci Rep. 2017;7(1):2409. doi:10.1038/s41598-017-02217-x DeCoste NJ, Gadkar VJ, Filion ...
qPCR analysis for gene expression is also sometimes called RT-qPCR since reverse transcription generates the cDNA for amplification. It has been observed that reverse transcription introduces most of the variability in mRNA measurement. Properties of the reverse transcription reaction in mRNA quantification...
Here, Droplet Digital PCR (ddPCR) and qPCR platforms were directly compared for gene expression analysis using low amounts of purified, synthetic DNA in well characterized samples under identical reaction conditions. We conclude that for sample/target combinations with low levels of nucleic acids (Cq...
01 水解探针 水解探针，也叫TaqMan或5'核酸酶，是最常用的探针类型之一，水解探针包括一个序列特定的...
[7] Zachar V, Thomas RA, Goustin AS. Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples. Nucleic Acids Res. 1993;21(8):2017‐2018. doi:10.1093/nar/21.8.2017 [8] Livak KJ, Schmittgen TD. Analysis of relative gene expression data usin...
Selection of reference genes for gene expression studies in porcine skeletal muscle using SYBR green qPCR. J Biotechnol 2010; 150: 288-93.Feng X., Xiong Y., Qian H., Lei M., Xu D., Ren Z. Selection of reference genes for gene expression studies in porcine skeletal muscle using SYBR ...
Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611-622. doi:10.1373/clinchem.2008.112797 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time ...
Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611-622. doi:10.1373/clinchem.2008.112797 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantita...
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 22DDCT Method[J...