Taylor SC, Laperriere G, Germain H. Droplet digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Sci Rep. 2017;7(1):2409. doi:10.1038/s41598-017-02217-x DeCoste NJ, Gadkar VJ, Filion ...
Taylor SC, Laperriere G, Germain H. Droplet digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Sci Rep. 2017;7(1):2409. doi:10.1038/s41598-017-02217-x DeCoste NJ, Gadkar VJ, Filion M. Relative and absolute ...
5. 最后我们看看最重要的结果,也就是目的基因的变化情况了。在内参与对照组选择没错的情况下,点击 gene expression 选项,选中你想查看的样品孔的基因表达情况。
01水解探针 水解探针,也叫TaqMan或5'核酸酶,是最常用的探针类型之一,水解探针包括一个序列特定的荧光...
Analysis of candidate reference genes by genorm The stability of potential reference genes was first examined via geNorm software and the expression stability (M) of all eight candidates was calculated. A candidate gene with an M value <1.5 is considered to be a good reference gene for qPCR37....
Reference Gene Selection for qPCR Gene Expression Analysis of Rust-Infected Wheat [J],Physiological and Molecular Plant Pathology, 2013, 81(4) : 22 - 25.Scholtz, J.J.; Visser, B. Reference gene selection for qPCR gene expression analysis of rust-infected wheat. Physiol. Mol. Plant Pathol....
实时荧光定量PCR(qPCR)是一种广泛应用于分子生物学和临床诊断的核酸定量技术。它的基本原理在于利用PCR...
Zhang H, Guan ZS, Guan SH, et al. Identification of Suitable Candidate Reference Genes for Gene Expression Analysis by RT-qPCR in Peripheral Blood Mononuclear Cells of CHB Patients. Clin Lab. 2016;62(1-2):227-234. doi:10.7754/clin.lab.2015.150805 ...
Here, Droplet Digital PCR (ddPCR) and qPCR platforms were directly compared for gene expression analysis using low amounts of purified, synthetic DNA in well characterized samples under identical reaction conditions. We conclude that for sample/target combinations with low levels of nucleic acids (Cq...
Real-time RT-PCR是qPCR和RT-PCR的组合,其中的“RT”是Reverse transcription(逆转录)的意思,所以RT-qPCR就是结合了荧光定量技术的逆转录PCR,即以mRNA或总RNA为模板,先反转录得到cDNA,再以cDNA为模板,通过荧光定量PCR进行定量检测分析。因为RT-PCR只可以定性,但不能进行定量分析。与RT-PCR一样,RT-qPCR定量分析...