To maximise the ultimate utility of liquid biopsy testing we have developed a highly versatile approach to NGS: Adaptor Template Oligo Mediated Sequencing (ATOM-Seq). ATOM-Seq'sstrengths and versatility avoid the major limitations ofboth PCR- and ligation-based approaches. This technology is ...
adapterqualitybioinformaticsquality-controlfilterngssequencingoverlapsplittingduplicationumitrimmingpreprocessingfilteringilluminafastqmergingqcpolyg UpdatedNov 28, 2024 C++ liyupi/sql-father-frontend-public Star1.5k Code Issues Pull requests 新项目:快速生成 SQL 和模拟数据的网站(React 前端),大幅提高开发测试效率!
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To maximise the ultimate utility of liquid biopsy testing we have developed a highly versatile approach to NGS: Adaptor Template Oligo Mediated Sequencing (ATOM-Seq). ATOM-Seq's strengths and versatility avoid the major limitations of both PCR- and ligation-based approaches. This technology is ...
Cogent NGS Analysis Pipeline (CogentAP) maps and reports RNA-seq data for full-length UMI-based or UMI-agnostic transcriptome analysis. Included are gene and transcript counting, gene fusion detection, and immune profiling analysis. The Cogent NGS Discovery ...
SMARTer Human TCR a/b Profiling Kit v2 (TCRv2)是一款具有更高重复性和特异性的TCR NGS文库构建试剂盒。该试剂盒整合SMART全长cDNA合成技术与5’RACE技术,可捕获TRA和TRB基因完整的V(D)J可变区,用于后续NGS分析。 免疫组库(Immune Repertoire,IR)是指在任何指定时间,个体的循环系统内所有功能多样性B细胞和T细...
Adaptor Template Oligo-Mediated Sequencing (ATOM-Seq): A versatile and ultra-sensitive UMI-based NGS library preparation technology, for use with cfDNA and cfRNA Liquid biopsy testing utilising Next Generation Sequencing (NGS) is rapidly moving towards clinical adoption for personalised oncology. However...
The sequencing runs were spiked with Illumina PhiX bacteriophage genome PhiX Control v3 in a concentration of 30% to increase the diversity which is required by these modern NGS machines. [41, 42] Sample de-multiplexing based on Illumina indices was carried out during FASTQ gen- eration and ...
44. UMI-based expanded NGS panel in precision molecular diagnosis of vascular anomalies: Early resultsdoi:10.1016/j.cancergen.2024.08.046Purpose To describe early results from a highly sensitive genetic panel to evaluate patients with largely mosaic vascular anomalies Methods This is a single-center ...
The recent use of Unique Molecular Identifiers (UMI) in NGS experiments offered a way to accurately separate true variants from artifacts. UMI-based variant callers are slowly replacing raw-reads based variant callers as the standard method for an accurate detection of variants at very low ...