Using a circularization-based concatemeric consensus sequencing approach (R2C2) paired with UMIs (R2C2 + UMI), we show that we can sequence an 550-nt antibody heavy chain (Immunoglobulin heavy chain - IGH) and an 1,500-nt 16S amplicons at accuracies up to and exceeding Q50 (<1 error ...
UMI-based RNA counting and full-length transcriptome analysis from single-cell and ultra-low inputsRNA sequencing (RNA-seq) captures the transcriptomic repertoire and quantifies the expression levels of genes. When performed on single cells (scRNA-seq), it...
1.Chung J, Lee K W, Lee C, et al. Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier[J]. BMC genomics, 2019, 20(1): 216. 2.Wang T T, Abelson S, Zou J, et ...
Abstract The diagnostic landscape of brain tumors integrates comprehensive molecular markers alongside traditional histopathological evaluation. DNA methylation and next-generation sequencing (NGS) have become a cornerstone in central nervous system (CNS) tumor classification. A limiting requirement for NGS and...
uBiome's commercial products include SmartGut, a sequencing-based clinical microbiome test, which identifies microbes in the gut for patients with chronic gut conditions such as IBD, IBS, Crohn's Disease, and ulcerative colitis; SmartJane, a sequencing-based women's health screening test, which ...
Here we present a straightforward unique molecular identifier (UMI)-linked consensus sequencing workflow (UMIC-seq) that simplifies mapping of evolutionary trajectories based on full-length sequences. Attaching UMIs to gene variants allows accurate consensus generation for closely related genes with ...
This UMI pipeline is based on Fulcrum Genomics toolkit, processes sequencing reads with molecular barcodes (also known as Unique Molecular Indices, UMIs),which provide impressive error correction and increased accuracy using a sequencing consensus read level. Input/Output file options --in-fq [IN_...
The sequencing error rate can be predetermined. The mismatch probability can be predetermined. In some embodiments, performing probability-based merging comprises: family identification and merging (or collapsing). Performing probability-based merging can comprise: duplex identification and merging (or ...
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