Basic Protocol 1: STARR‐seq plasmid library cloning Basic Protocol 2: Mammalian STARR‐seq screening protocol Alternate Protocol: UMI‐STARR‐seq screening protocol—unique molecular identifier integration Support Protocol: Transfection of human cells using the MaxCyte STX scalable transfection system...
UMI-STARR-seq (Alternate Protocol; right): For low-complexity candidate libraries, unique molecular identifiers (UMI) can be introduced prior to amplification, allowing the precise counting of reporter mRNAs (Alternate Protocol). To render the reporter cDNAs competent for UMI introduction, second-...