The rapid accumulation of the whole genome sequencing (WGS) and the whole exome sequencing (WES) data has led to the discovery of a tremendous number of genetic variations, both pathogenic and non-pathogenic. To aid the assessment and understanding of these variations, population databases such ...
CPM-TMM normalized gene counts across technical replicates for a given biological replicate were averaged for the count tables used in all analyses performed. Sequencing batches and plasma volumes were obtained from the authors in Toden et al. and Chalasani et al. for per-sample normalization. For...
DNA sequencing of these clones showed that the type 3 gene was 2207 nucleotides long and the single long open reading frame encoded a primary translation product (渭1) of 709 amino acids with a molecular weight of 76,000. The type 1 gene was three nucleotides shorter at 2204 with the ...
Single-cell messenger RNA sequencing reveals rare intestinal cell types. Understanding the development and function of an organ requires the characterization of all of its cell types. Traditional methods for visualizing and isol... Dominic,Grün,Anna,... - 《Nature》 被引量: 356发表: 2015年 ...
are located close to one another on the samechromosomeand tend to be inherited together. Such linkage groups can be used to identify unknown genes that influence disease risk. Technological advances, especially inDNA sequencing, have greatly increased the catalogue of variable sites in thehuman ...
Single-Cell RNA Sequencing Reveals the Cell Types Heterogenicity of Human Discoid Lateral Meniscus Cells Single-Cell RNA Sequencing Reveals the Cell Types Heterogenicity of Human Discoid Lateral Meniscus Cellsdoi:10.2139/ssrn.3761110 H Sun,W Zhang,F Liu,... - 《Social Science Electronic Publishing...
ACTINN: Automated Identification of Cell Types in Single Cell RNA Sequencing. Bioinformatics https://doi.org/10.1093/bioinformatics/btz592 (2019). Hou, R., Denisenko, E. & Forrest, A. R. R. scMatch: a single-cell gene expression profile annotation tool using reference datasets. Bioinformatics...
Single-cell RNA-sequencing (scRNA-seq) has emerged as a key technology for profiling the gene expression program at both the global transcriptome level and at the single-cell resolution. Effective computational analyses of scRNA-seq data is essential for extracting underlying biological knowledge from...
The HPV L1 gene was amplified by PCR using original and modified GP5+/6+ primers. Amplified PCR products were genotyped by the microarray system GeneSquare (KURABO) and/or clonal sequencing. Of the 281 women, 139 (49.5%) were positive for HPV DNA. Among the HPV-positive samples, 339 ...
Analyzing single-cell bisulfite sequencing data with MethSCAn ArticleOpen access31 July 2024 Main Understanding how the same DNA sequence is interpreted differently in different cell types is a fundamental challenge of biology. Gene expression, DNA accessibility and chromatin packaging are well-established...