Bacterial DNA methylation occurs at diverse sequence contexts and plays important functional roles in cellular defense and gene regulation. Existing methods for detecting DNA modification from nanopore sequencing data do not effectively support de novo study of unknown bacterial methylomes. In this work, ...
Bacterial DNA methylation occurs at diverse sequence contexts and plays important functional roles in cellular defense and gene regulation. Existing methods for detecting DNA modification from nanopore sequencing data do not effectively support de novo study of unknown bacterial methylomes. In this work, ...
To achieve DNA sequencing with solid-state nanopores, the speed of the DNA in the nanopore must be controlled to obtain sequence-specific signals. In this study, we fabricated a nanopore-sensing system equipped with a DNA motion controller. DNA strands were immobilized on a Si probe, and appro...
Our approach is complementary to previous work using cell-free nucleosomes14, which depends on a more limited set of reference chromatin immunoprecipitation sequencing data, which are largely at the tissue level43. Readily measurable cell types include those specific to the brain, lung, intestine, ...
Here, we present an in-depth analysis of 54 single-CpG-resolution DNA methylomes of normal human cell types by integrating high-throughput sequencing-based methylation data. We found that the ratio of methylated to unmethylated CpGs is relatively constant regardless of cell type. However, which ...
and a population ofPdgfrα+fibroblasts in the mouse trachea induced differentiation of basal cells through the production of IL626. In the distal lung,Axin2+Pdgfrα+fibroblasts support AT2 cells through Il6, Bmp, and Fgf signaling modulation27. Using single-cell RNA sequencing (scRNA-seq), Tsuk...
The two types of Drosophila melanogaster rDNA insertion occur at different sites in the 28S gene, separated by 51 nucleotide pairs. A short segment of rDNA... H Roiha,JR Miller,LC Woods,... - 《Nature》 被引量: 277发表: 1981年 Sequencing of Pooled DNA Samples (Pool-Seq) Uncovers Com...
Analyzing single-cell bisulfite sequencing data with MethSCAn ArticleOpen access31 July 2024 Main Understanding how the same DNA sequence is interpreted differently in different cell types is a fundamental challenge of biology. Gene expression, DNA accessibility and chromatin packaging are well-established...
To achieve DNA sequencing with solid-state nanopores, the speed of the DNA in the nanopore must be controlled to obtain sequence-specific signals. In this study, we fabricated a nanopore-sensing system equipped with a DNA motion controller. DNA strands were immobilized on a Si probe, and appro...
Imaging of fluorescently labeled DNA in single cells validates these subtypes and shows radial positioning of LADs with higher LAMIN B1 occupancy and heterochromatic histone modifications primarily embedded within the lamina. In contrast, the second subtype of lamina-associated chromatin is relatively gene...