Describe the DNA . Describe how to construct a recombinant DNA. Describe the uses of DNA ligase in cells. Describe the process of Sanger sequencing using capillary electrophoresis. Describe DNA structure. Describe the basic process of DNA repair. ...
Describe the function of DNA polymerase III. Describe the three stages of Transcription in detail. What is Sanger sequencing? Explain how it works and how the PCR process is different. Describe the steps for DNA processing. Explain why you need two primers for PCR and what would happen if y...
that might also be biased by uneven DNA amplification across organisms23,24. Although multiple primer sets targeting different genome regions could be used to overcome these problems14,25, shotgun sequencing of all DNA, without any preliminary selection, has the potential to ...
High-throughput technologies like microarray profiling or next generation sequencing enable us to examine the validity of the con- cept, by profiling transcriptomes and multiple chromatin marks for many different samples, conditions and organ- isms. The large amounts of generated data requi...
The most commonly used clinical diagnostic methods are still based on the methods introduced by Pasteur in the 1880s. Culture-based technology is time-consuming and has a low positive rate. Metagenomic second-generation sequencing (mNGS) technology is used as an unbiased DNA/RNA detection method ...
Nucleotide sequencing data of DQfl genomic clone (DQfiDwl2) from the same HLA-Dw12 homozygous individual indicated that the acceptor site was intact (AGG). Restriction enzyme AvaII could cleave the intact site (AGGACC), but not the aberrant site (AAGACC), so that this base substitution ...
The most commonly used clinical diagnostic methods are still based on the methods introduced by Pasteur in the 1880s. Culture-based technology is time-consuming and has a low positive rate. Metagenomic second-generation sequencing (mNGS) technology is used as an unbiased DNA/RNA detection method ...
The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown. In this work, we performed data mining of TF–glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq)...
Molecular methods of species delineation are rapidly developing and widely considered as fast and efficient means to discover species and face the 'taxonomic impediment’ in times of biodiversity crisis. So far, however, this form of DNA taxonomy frequen
Molecular methods of species delineation are rapidly developing and widely considered as fast and efficient means to discover species and face the 'taxonomic impediment’ in times of biodiversity crisis. So far, however, this form of DNA taxonomy frequen