Sanger sequencing utilizes a chain-termination method to provide the identity and order of nucleotide bases in a given strand of DNA. This method makes use of chemical analogues of the four nucleotide bases. These analogues, called ddNTPs, are missing the hydroxyl group ...
关键词:基准与方法研究;基因测序;变异检测;文献简介标题(英文):Standardized Comparison of Different DNA Sequencing Platforms标题(中文):不同 DNA 测序平台的标准化比较发表期刊:Clinical Chemistry…
Additionally, the DNA methylation profiles of the samples were determined by reduced representation bisulphite sequencing (RRBS). Results In this study, different traits related to sperm DNA integrity were investigated (n = 18 ejaculates). Upon liquid storage, the levels of total thiols and ...
DNA bisulfite modification and sequencing of target amplicons were used to determine the CpG methylation status of HPV16, HPV18 and HPV51 genomes at 3′ part of L1 gene and LCR sequences in DNA samples purified from a collection of cervical specimens with confirmed HPV infection (n=202). Compl...
DNA sequencing and assembly DNA libraries were prepared with insert size of 500 bp and were sequenced using the Illumina Genome Analyzer (Illumina). We used 2 × 50 bp paired end sequencing. Contigs were assembled using the Short Oligonucleotide Analysis package (SOAP) (version 1.04) [12]. SOA...
L. et al. The human pancreatic islet transcriptome: expression of candidate genes for type 1 diabetes and the impact of pro-inflammatory cytokines. PLoS Genet. 8, e1002552 (2012). This study provided the first RNA sequencing study of cytokine-stressed human β-cells and showed that >50% of...
What is DNA sequencing? The molecular structure of DNA is also called ___. How does DNA replicate itself? DNA and RNA are both made of parts called. Which one is genetic material, DNA or RNA? What is a complex of DNA and proteins that constitutes eukaryotic chromosomes...
In recent years, advantages in the whole genome analysis through development of next-generation sequencing techniques, allowed also very detailed analysis of mtDNA mutational spectra in various tissues. These studies demonstrated that mtDNA mutations indeed increase with increasing age [23], [24], [25...
(MTIs)44. It provides diverse information on miRNAs which probably influence the submitted genes. It is widely used to detect transcriptome-affecting miRNAs, and its data are validated by experimental methods such as molecular assay, Northern blot, Microarray, and next-generation sequencing (NGS)45...
Cucumber, Cucumis sativus L., is an economically and nutritionally important crop of the Cucurbitaceae family and has long served as a primary model system for sex determination studies. Recently, the sequencing of its whole genome has been completed. Ho