We optimized TurboID-based proximity labeling inN. benthamianato enable identification of protein interactions partners (Supplementary Fig.3).N. benthamianaplants were infiltrated with agrobacterium containing the biotin ligase-fusion construct and at 36 hpi biotin was infiltrated and the plants were incubat...
Introduction Protocol Representative Results Discussion Acknowledgements Materials References English Explore More Videos Keywords TurboID Proximity Labeling Protein protein Interactions NLR Immune Receptors Nicotiana Benthamiana Agroinfiltration Biotin RIPA Lysis Buffer ...
文献阅读-1:Exploiting effector-host interactions using TurboID-based proximity labeling 1只小脑斧ya 总要坚持些什么吧1 人赞同了该文章 科学问题:Ustilago maydis效应子UmSee1是玉米感染期间叶片中肿瘤形成所必需的。UmSee1与玉米SGT1相互作用并阻断其在体内的磷酸化。然而,目前尚不清楚哪些宿主过程被UmSee1和Um...
Biotin labelingU251 cellshnRNPKProteinprotein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than BioID or APEX that reduces the required labeling time from 18h to 10min. Since many proteins participate in binding...
TurboID Proximity Labeling of a Protocadherin Protein to Characterize Interacting Protein Complex The study of the neuron has always been a fundamental aspect when it came to studying mental illnesses such as autism and depression. The protein protocadh... C Wang,L Yu - 美国分子生物学期刊(英文)...
To overcome the drawbacks of traditional approaches for analyzing PPIs, enzyme-catalyzed proximity labeling (PL) techniques based on peroxidases or biotin ligases have been developed and successfully utilized in mammalian systems. However, the use of toxic H2O2 in peroxidase-based PL, the requirement ...
TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity. Nature Communications. 10 (1), 3252 (2019). Arora, D., et al. Establishment of proximity-dependent biotinylation approaches in different plant model systems. bioRxiv. , (2019). Kim,...
Proximity labelling provides a powerful in vivo tool to characterize the proteome of sub-cellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labelling protocol for C. elegans . We use this protocol to characterise the ...
The current study was designed to identify potential regulators of IRE1 function by employing the TurboID proximity labeling technique to discern IRE1α and IRE1β interaction proteomes in the HMC-1.2 cell line. Therefore, IRE1α-deficient HMC-1.2 (HMC-1.2IRE1α−/−) cells were stably ...
The Ti‐TAN plasmid toolbox for TurboID‐based proximity labeling assays in Nicotiana benthamianadoi:10.1111/jipb.13610Tan, HuangZhou, YuDinius, ErikLozano‐Durán, RosaJournal of Integrative Plant Biology