Immunology CD4+ T cell mediated tumor immunity following transplantation of TRP-1 TCR gene modified hematopoietic stem cells INDIANA UNIVERSITY Christopher E. Touloukian HaSung PilImmunotherapy for cancer has h
CD4+T cells were isolated using MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend, China, # 480,039). Naïve CD4+T cells were cultured in OptiVitro® T Cell Serum-Free medium (ExCell Bio) supplemented with 1 μg/ml αCD28 (BioLegend, China, #102,103) and differentiat...
Furthermore, TRPV1 activation is also involved in regulating immune cell activity, including the activation of CD4-positive T cells and the proinflammatory phenotype polarization of macrophages (M1)16,17. In contrast, a recent study has shown that TRPV1 modulates M1/M2 macrophage polarization and...
TRPV1 neurons affect the recruitment of immune cells. TRPV1 inhibited the recruitment of neutrophils. After using RTX to chemically denervate and ablate TRPV1+ neurons, RTX-treated mice displayed greater lung neutrophil recruitment and higher CD4 T cells number.[69] Similarly, TRPV1 KO mice ...
13,16 Previous study has revealed that TRPV1 is functionally expressed in CD4+ T cells and promotes the activation and pro-inflammatory properties of CD4+ T cells.14 The wide distribution of TRPV1 in immune cells suggests its role in modulating inflammatory responses. However, the reported ...
(Fig.2h). Immunostaining of the hippocampus showed prominent interactions between Iba1+microglia and CD4 T cells or CD8 T cells (Fig.2h). These observations indicated thatAPOE4influences the phagocytic capacity and antigen presentation by microglia, thus resulting in the priming of the adaptive ...
Later, TRPV1 expression was reported in non-neuronal system cells, such as arteriolar smooth muscle cells [13, 14] and the bladder urothelium [15]. TRPV1 is a nonselective cation channel that can be activated by different physical and chemical stimuli, including temperatures over 43°C, ...
Allergen-specific CD4+ T cells are regarded as pivotal in inflammation, resulting in the infiltration of eosinophils and the activation of mast cells, followed by tissue remodeling, excessive airway mucus secretion, and airway hyperresponsiveness3. Though it is well known that immune mechanisms play ...
Figure 3. TRPV1+ Neuron Activation Induces Expansion of IL-17-Producing TCRγδ and CD4 T Cells (A and B) Representative flow-cytometry plots (A) and summary data (B) of IL-17A expression in phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulated ILC, TCRγδ, and CD4 T cells iso...
T cells by controlling the T cell receptor signaling complex44. The activation of TRPV1 also stimulates extracellular-signal-regulated, c-Jun N-terminal, and p38 mitogen-activated protein kinases and releases the cytokines IL-6 and -8 in a manner that is dependent on extracellular Ca2+levels ...