TROSY HSQCNMR assign histidine tautomer proteinBachovchin, W W
Provided that care is taken in adjusting the WATERGATE element of a (1)H-(15)N TROSY-HSQC experiment, such that neither the water magnetization nor the (1)H(±) protons are inverted by its final 180° pulse, (3)JHNH± couplings can be measured directly from splittings in the (1)H ...
Measurements are based on quantitative analysis of intensities in 1H–15N and 13C–1H TROSY-HSQC spectra, and are illustrated for a 71-nucleotide adenine riboswitch. Results compare favorably with those of conventional frequency-based measurements in terms of completeness and convenience of ...
Isotopically discriminate IDIS-HSQC TROSY NMR for complex protein interaction in vitroGolovanov, A PBlankley, R TAvis, J MBermel, W
Superposition of (1H-15N) TROSY-HSQC-spectra of OcDH-His5 after titration with different substrate concentrations.Sander, H. J. SmitsTatu, MeyerAndre, MuellerNadine, van OsMatthias, StoldtDieter, WillboldLutz, SchmittManfred, K. Grieshaber
The advantages of this method compared to conventional HSQC include increased spectral resolution, increased sensitivity, and reduced linewidths. TROSY may be further improved using CRINEPT (cross-relaxation-enhanced polarization transfer), which improves magnetization transfer efficiency in very large ...
Clean SEA-HSQC: A method to map solvent exposed amides in large non-deuterated proteins with gradient-enhanced HSQC The recent introduction of the SEA-TROSY experiment (Pellecchia et al. (2001) J. Am. Chem. Soc., 123, 4633-4634) can alleviate the problem of resonance ove... D Lin,HS ...
in isotopically enriched proteins - Hu, Bax - 1997 () Citation Context ...re measured using HSQC-NOESY-TROSY experiments with a mixing time of 150 ms... HA Bax - 《Journal of Biomolecular Nmr》 被引量: 54发表: 1997年 加载更多来源...
A new and convenient method, named ARTSY-j, is introduced that permits extraction of the (3)J(HNH alpha) couplings in proteins from the relative intensities in a pair of N-15-H-1 TROSY-HSQC spectra. The pulse scheme includes (3)J(HNH alpha) dephasing of the narrower TROSY H-1(N)-...
The ARTSY method derives the size of the coupling from the ratio of intensities observed in two TROSY-HSQC spectra recorded with different dephasing delays, thereby minimizing potential resonance overlap problems. Precision of the RDC measurements is limited by the signal-to-noise ratio, S/N, ...