1H-15N TROSY-HSQC spectrum of Gαi1 shows extensive peak broadening and intensity loss upon binding of Ric-8A.Celestine, J. ThomasKlára, BriknarováJonathan K., HilmerNavid, MovahedBrian, BothnerJohn P., SumidaGregory G., TallStephen R., Sprang...
After an additional hour at 37 °C, expression was induced with the addition of 1 3Vol.:(0123456789) 1 80 Fig. 1 Left 1H–15N TROSY HSQC spectrum of YAP 50–171 at pH 6 and 298 K. Right magnification of the cen- tral region of the spectrum M. Feichtinger et al...
Superposition of (1H-15N) TROSY-HSQC-spectra of OcDH-His5 after titration with different substrate concentrations.Sander, H. J. SmitsTatu, MeyerAndre, MuellerNadine, van OsMatthias, StoldtDieter, WillboldLutz, SchmittManfred, K. Grieshaber
The dynamic NOE measurements can be readily applied for non-deuterated or deuterated proteins in both HSQC and TROSY-type experiments. Comparison of the dynamic NOE method with commonly implied steady-state NOE is presented in measurements performed at three magnetic field strengths. It is also ...
the measurement of 1 J HN, 1 J NC′, and 2 J HC′ coupling constants [J. Am. Chem. Soc. 125 (2003) 11504] was modified to yield a double-TROSY experiment which selects 1 of the 16 multiplet components from a 15N-HSQC spectrum recorded of a uniformly 15N/ 13C-labelled protein. ...
Backbone 1H, 13C, and 15N resonances were assigned using a combination of automated and manual methods with data from the following experiments: 2D 15N HSQC, 2D TROSY, 3D trHNCO, tr HNCACO, trHNCA, trHNCOCA, trHNCACB, trHNCOCACB, 15N-edited NOESY-HSQC (mixing time, 60 ms), and 15N...
A 3D experiment simultaneously provides the spectra of a 3D NOESY-HN-TROSY and of a 3D NOESY-HC-PEP-HSQC. Thus, nOes from any protons to methyl or amide protons are dispersed with respect to 15 N and 13 C chemical shifts, respectively. The single 4D experiment presented here yields ...
resonance assignments for domain R2 (residues 327-443) were obtained with 15N, 13C/15N and 2H/13C/15N-labeled protein utilizing 3D CT-HNCA, CT-HN(CO)CA, TROSY- HNCO, TROSY-HN(CA)CO, TROSY-HNCACB, TROSY-HN(CO)CACB, and 4D 15N/15N-NOESY, TROSY-HNCOCA, and TROSY-HNCACO ...