1H-15N TROSY-HSQC spectrum of Gαi1 shows extensive peak broadening and intensity loss upon binding of Ric-8A.Celestine, J. ThomasKlára, BriknarováJonathan K., HilmerNavid, MovahedBrian, BothnerJohn P., SumidaGregory G., TallStephen R., Sprang...
Resonances assignment for hPARP14 MD2 in the free and in the ADPr bound form was achieved analyzing the following series of heteronuclear experiments: 2D [1H,15N]–HSQC and 2D [1H,15N]–TROSY, 3D HN(CO)CA, 3D HNCA, 3D TROSY HN(CO)CACB, 3D TROSY HNCACB, 3D HN(CA)CO, 3D HNCO, ...
A series of 2D [1H, 15N]-HSQC experiments were also recorded using the samples labeled by residue type. Sidechain resonances were assigned using 3D CC(CO)NH, 3D (H)CC(CO)NH, 3D HNHA, 3D HBHA(CO)NH, 3D 15N-edited NOESY-TROSY, 3D 15N-edited TOCSY- TROSY, 3D 13C-edit...
Superposition of (1H-15N) TROSY-HSQC-spectra of OcDH-His5 after titration with different substrate concentrations.Sander, H. J. SmitsTatu, MeyerAndre, MuellerNadine, van OsMatthias, StoldtDieter, WillboldLutz, SchmittManfred, K. Grieshaber
4 Figure 1: (Top) 1H-15N TROSY spectrum of the N-terminal domain of PilB from Neisseria meningitidis at 298 K and 600 MHz. (Bottom) Sequences alignment of the N- terminal domain of PilB from Neisseria meningitidis (with secondary structure elements predicted by TALOS) with...
Sklenár V, Peterson R, Rejante M, Feigon J (1994) Correlation of nucleotide base and sugar protons in a 15N-labeled HIV-1 RNA oligonucleotide by 1H–15N HSQC experiments. J Biomol NMR 4:117–122. doi:10.1007/BF00178339 Google Scholar Sripakdeevong P, Cevec M, Chang AT, Erat MC,...
The dynamic NOE measurements can be readily applied for non-deuterated or deuterated proteins in both HSQC and TROSY-type experiments. Comparison of the dynamic NOE method with commonly implied steady-state NOE is presented in measurements performed at three magnetic field strengths. It is also ...
Backbone 1H, 13C, and 15N resonances were assigned using a combination of automated and manual methods with data from the following experiments: 2D 15N HSQC, 2D TROSY, 3D trHNCO, tr HNCACO, trHNCA, trHNCOCA, trHNCACB, trHNCOCACB, 15N-edited NOESY-HSQC (mixing time, 60 ms), and 15N...
resonance assignments for domain R2 (residues 327-443) were obtained with 15N, 13C/15N and 2H/13C/15N-labeled protein utilizing 3D CT-HNCA, CT-HN(CO)CA, TROSY- HNCO, TROSY-HN(CA)CO, TROSY-HNCACB, TROSY-HN(CO)CACB, and 4D 15N/15N-NOESY, TROSY-HNCOCA, and TROSY-HNCACO ...