1h、15n异核单量子相关(hsqc)1h、15n异核单量子相关(hsqc) 1H、15N异核单量子相关(HSQC)是一种核磁共振(NMR)实验技术,用于研究有机化合物中的氢和氮原子之间的相互作用。它是一种二维NMR实验,通过测量氢原子和相邻氮原子之间的化学位移差异,提供了有关分子结构和化学环境的信息。 在HSQC实验中,样品被置于一个...
在HSQC技术中,通过观察氢和氮原子之间的化学位移耦合,可以推断它们之间的化学结合和相互作用。下面是关于1H-15N异核单量子相关的相关参考内容。 1.原理和应用: - HSQC技术的原理是通过在一个频率维度上记录氢原子的化学位移,而在另一个频率维度上记录氮原子的化学位移,以获得氢和氮之间的关联信息。这种技术可以用于...
We report a detailed study of specificity, interaction and dissociation constants (Kd) of the peptide-ligand 螖-somatostatin (AGSKNFFWKTFTSS) binding to hPDI using19F ligand-observe and15N,1H-HSQC protein-observe NMR methods. Phe residues in 螖-somatostatin are hypothesised as important for ...
The first step is to combine an amide-proton relaxation period with a 15N-1H HSQC as a read-out for resolution. Torchia’s group showed that this can be done in four different ways [25]; the amide-proton relaxation period can precede the HSQC, or can be placed behind the HSQC; ...
It is shown that in certain circumstances the sensitivity of nitrogen-15-proton gradient-selected HSQC spectra of proteins dissolved in H O is compromised due to exchange between the NH and the water protons. The loss in sensitivity arises because, at the start of the relaxation delay, the mag...
HSQC实验在化学研究中的应用: 1.蛋白质结构研究:HSQC可以用于蛋白质的结构分析,特别是用于确定氨基酸残基之间的氢-氮关联。这对于理解蛋白质的折叠、相互作用和功能非常重要。 2.化合物鉴定:HSQC可以用于识别和鉴定有机化合物。通过观察特征的氢-氮相关信号,可以确定化合物中的结构、取代基、功能团等。 3.代谢组学...
3 Figure 1: (a) 1H-15N HSQC spectrum of uniformly 15N-labeled and partially deuterated Rac1 C178S K184Stop loaded with GMPPNP, collected at 800MHz (1H resonance frequency) at 25°C and pH 6.8. (b) Difference between experimental Cα chemical shift and random-coil chemical shif...
光诱导1H 15N HSQC化学位移选择的样本的扰动分析表明,在photoaccumulated 翻译结果2复制译文编辑译文朗读译文返回顶部 翻译结果3复制译文编辑译文朗读译文返回顶部 光致1H15N 异化学位移摄动分析抽取的样本显示,photoaccumulated 翻译结果4复制译文编辑译文朗读译文返回顶部 ...
有偿求助，蛋白质和小分子配体复合物15N-1H HSQC图谱解析
a来自周皋中学的英语 Comes from week high bank middle school's English [translate] aLight-induced 1H15N HSQC chemical shift perturbation analysis of selected samples indicated that the photoaccumulated [translate] aShort-distance information from 1H15N HSQC chemical shift [translate] ...