pLKO.1-TRC control载体价格1000元,addgene 10879, pLKO.1-TRC control载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为氨苄青霉素,质粒大小为7050bp,测序引物见下文。pLKO.1-TRC control载体是慢病毒载体pLKO.1-TRC control是RNAi载体。
Each clone contains a unique barcode sequence that can be easily identified, allowing for simple deconvolution of pooled screening experiments EF1a promoter drives expression of the ORF construct, offering an alternative to the CMV promoter which may not be appropriate in certain cell types Puromycin ...
As the focus is on trc promoter, the examples given exclusively cover trc expression vectors. The classification of expression vectors, according to transcription promoters, is somewhat arbitrary and has historical reasons originating from a time when a strong promoter was considered the most important...
Positive clones were sequenced using an SP6 promoter primer. Sequences of gene-specific primers, used in 5′RLM-RACE reactions, are presented in Table S3. Detection of Apoptosis Apoptosis in HCT116 cells was detected with an Annexin V-Cy3 apoptosis detection kit (BioVision) according to the ...
Human phosphoglycerate kinase promoter drives expression of puromycin. Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian cells. 3’ Self-inactivating long terminal repeat. f1 bacterial origin of replication. Ampicillin resistance gene for selection of pLKO.1 plasmid in bacterial ...
(1)GUUS expression vector. 35S and NOST are promoter and terminator for gene transcription respectively, two gray boxes (U) represent the direct repeats of intenal GUS gene, gRNA recognition and cleavage sequence represented by arrows. (2)GUSRec and the vector are expressed in one cell simultane...
Each clone contains a unique barcode sequence that can be easily identified, allowing for simple deconvolution of pooled screening experiments EF1a promoter drives expression of the ORF construct, offering an alternative to the CMV promoter which may not be appropriate in certain cell types Puromycin ...
4C). The reintroduction of INSIG1 with a native promoter and a synonymous encoding sequence in insig1 knockout cells restored the HIV-1 production to WT (Fig. 4, D and E). Therefore, the experiments of both the overexpression and the knockout of INSIG1 support the critical role of ...
Each clone contains a unique barcode sequence that can be easily identified, allowing for simple deconvolution of pooled screening experiments EF1a promoter drives expression of the ORF construct, offering an alternative to the CMV promoter which may not be appropriate in certain cell types Puromycin ...
The present invention provides the sequence for a novel gene called TRC8 which is located on chromosome 8. Various types of alterations in the gene have been shown to be associated with renal and thyroid tumors. One such alteration involves a 3;8 translocation which interrupts TRC8 and results...