T7 Promoter Primer features include: • Desalted and purified by gel filtration • Assayed for function by PCR amplification • Provided in 2 μg quantity Applications • Sanger sequencing • PCR amplification T7 Primer Sequence:5´- TAATACGACTCACTATAGGG- 3´ ...
Promoter for bacteriophage T7 RNA polymerase. Try SnapGene for Free |Download SnapGene Viewer Explore Over2.7kPlasmids:Basic Cloning Vectors|More Plasmid Sets Show Static Map 15105 End(19)SfcI(13)Start(0) T7 promoter T7 promoter19 bp
insectcellswithplasmidscontainingthegenesforchloramphenicolacetyltransferase(CAT),thehepatitisBvirusprecore-,core-ore-antigensundercontroloftheT7promoter,transientexpressionofthesegeneswasdetectedbyELISATheresultsobtainedindicatethatthisbaculovirus/T7systemprovidesasimpleandwidelyapplicabletoolfortransientgeneexpression...
("leaky") expression, which can be a drawback when recombinant proteins are toxic to the host cell. Therefore, our vectors contain the lac operator (lacO) sequences immediately downstream from the promoter to reduce leaky expression. Unlike the tac promoter system, pT7 vectors must be ...
T7 PROMOTER-BASED EXPRESSION SYSTEMAn improved T7 based promoter-driven protein expression system comprising an operator sequence downstream of the T7 promoter sequence, and having a further operator sequence upstream of the T7 promoter sequence....
T7 Promoter Primer features include:• Desalted and purified by gel filtration• Assayed for function by PCR amplification• Provided in 2 μg quantityApplications• Sanger sequencing• PCR amplificationT7 Primer Sequence: 5´- TAATACGACTCACTATAGGG- 3´ For Research Use Only. Not for...
activating gene expression in mammalian cells with promoter:激活启动子在哺乳动物细胞中的基因表达 热度: CAGE-TSSchip promoter-based expression profiling using the 5´-leading label of capped transcripts 热度: UCSC-Ensembl查找基因启动子(promoter)、内含子、外显子序列 ...
pGADT7 AD is a yeast expression vector that is designed to express a protein of interest fused to a GAL4 activation domain (AD; amino acids 768–881). Transcription of the GAL4 AD fusion is driven by the constitutively active ADH1 promoter (PADH1), and is terminated at the ADH1 transc...
transcription. Furthermore, the synthetic promoter isdesigned to perform constitutive or tissue or cell-type specific or temporal or inducible expression of gene system. A deep interrogation of thecis-regulatory architecture, which includesmotif sequence, position, copy number, and spacer length, ...
An autogene plasmid, pT7-G1, containing the T7 phage RNA polymerase-encoding modified gene (G1) under control of its cognate T7 promoter, was only obtained when the plasmid contained the encephalomyocarditis (EMC) untranslated sequence. In vitro transcription and translation studies confirmed that...