insectcellswithplasmidscontainingthegenesforchloramphenicolacetyltransferase(CAT),thehepatitisBvirusprecore-,core-ore-antigensundercontroloftheT7promoter,transientexpressionofthesegeneswasdetectedbyELISATheresultsobtainedindicatethatthisbaculovirus/T7systemprovidesasimpleandwidelyapplicabletoolfortransientgeneexpression...
T7 Expression System The T7 expression system allows high-level expression from the strong bacteriophage T7 promoter. It’s ideal for expressing soluble, nontoxic recombinant proteins inE. coli.The T7 expression vectors are designed to facilitatecloningusingGateway®technology,...
pT7-FLAG®and pT7-MAT™ vectors offer the very strong T7/lac promoter. These expression vectors produce even higher yields of recombinant protein than the tac promoter system. However, the T7 promoter is known for background ("leaky") expression, which can be a drawback when recombinant...
1.pET Bacterial Recombinant Protein Vector.https://en.vectorbuilder.com/learning-center/vector-system/pET16b.html 2.J.W.Dubendorff,F.W.Studier.Controlling Basal Expression in an Inducible T7 Expression System by Blocking the Target T7 Promoter With Lac Repressor. J Mol Biol. 1991 May 5;219...
WO1999005297A1 1998年7月21日 1999年2月4日 Zeneca Limited T7 promoter-based expression systemKara, B.V., Pioli, D., Bundell, K.R, and Hockney, R.C. T7 promoter-based expression system. [6537779]. 25-3-2003. USA.WO1999005297A1 Jul 21, 1998 Feb 4, 1999 Zeneca Limited Systeme d'...
protein expression systemWe constructed T7 expression systems in Bacillus subtilis, in which the rhamnose-inducible rhaEW promoter and its derivative were used to control the T7 RNA polymerase gene. This promoter is regulated by the RhaR repressor, which is derepressed by rhamnulose-1-phosphate ...
表達系統(T7expressionsystem)。T7表達系統包含T7RNA聚合酉每(T7RNA polymerase)和一個能被T7RNA聚合酉每驅動的T7啟動子(T7promoter)。T7RNA聚合酉每 是噬菌體(phage)T7基因1(T7gene1)的產物,相較於大腸桿菌的RNA聚合酉每,它對於選 殖基因(clonedgene)具有極為優越的轉錄(transcription)能力(Colomband ...
Bacteriophage T7 lysozyme, a natural inhibitor of T7 RNA polymerase, can reduce basal activity from an inducible gene for T7 RNA polymerase and allow relatively toxic genes to be established in the same cell under control of a T7 promoter. Low levels of T7 lysozyme supplied by plasmids pLys...
To investigate the effects of nuclear localization signal on immune response induced by eukaryonizedT7 expression system. 研究核定位信号对真核化的T7表达系统在真核细胞及基因免疫中表达HBsAg基因效率的影响。 更多例句>> 2) T7 promoter system
2) T7 promoter T7启动子 1. Construction of expression system controlled by T7 promoter at different expression level in mammalian cells; 在哺乳类动物细胞中呈现不同表达水平的T7启动子表达系统构建 2. Methods cDNA of TIMP-1 was obtained by RT-PCR,and coloned PGEM-T vector,and identified ...