Leaky gene expression occurs when a genetic promoter is not fully repressed, leading to unintended protein synthesis in the absence of stimuli. Existing work has devised specific molecular strategies for reducing leaky gene expression of each promoter. Main Method and Results: In contrast, we ...
("leaky") expression, which can be a drawback when recombinant proteins are toxic to the host cell. Therefore, our vectors contain the lac operator (lacO) sequences immediately downstream from the promoter to reduce leaky expression. Unlike the tac promoter system, pT7 vectors must be ...
PromoterInducerProperties araArabinoseRapid induction, strong regulation, repressed by glucose CadAcidic pHHigh expression levels, simple induction process but the pH may adversely affect the producer LacIsopropyl-β-d-thiogalactoside (IPTG)Relatively low-level expression, leaky expression under noninducing ...
In those cases, a leaky premature expression of the phage Pol gene in the presence of a T7 promoter–target gene is likely to render the cells unviable before reaching the stage of full expression. This problem could be resolved in principle if the T7 RNA Pol is expressed under a “...
leaky promoters (see below) in the same cell comprising inducing expression of the genes only when the cells are in logarithmic growth phase, or stationary phase, and isolating the polymerase from the cell; preferably the cloned fragments are under the control of a promoter requiring T7 RNA ...
Using various T7 RNA polymerase/T7 promoter-based vector host systems with differential control on expression of the T7 RNA polymerase, we document that leaky expression of the latter is responsible for the frequently observed loss of the culture's ability to express genes of interest. We ...
The induction of T7 promoter with isopropyl-尾-d-thiogalactopyranoside (IPTG) is widely used for expression of large quantities of proteins in Escherichia coli. It has been reported that basic T7 promoter is leaky and expresses cloned genes without induction. The effect of T7 promoter induction...
This transposon contains the T7 RNA polymerase driven by the lacUV5 promoter, which is repressed by the lac-repressor. Leaky expression is prevented by the presence of T7-lysozyme on this construct. The complete T7-expression system is flanked by mariner transposon repeats of the suicidal R6K...
A method of producing active T7-type DNA polymerase from\ncloned fragments. comprising placing the genes encoding said\npolymerase under the control of non-leaky promoters in a single\ncell, inducing expression of said genes when said cell is in\nlogarithmic growth phase, or stationary phase, ...
LEAKY PROMOTERGENE-10We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct the pARHS vectors. These vectors are stable for at least 60 cell ...