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T7 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T7 DNA or DNA cloned downstream of a T7 promoter. Although the T7 and T3 promoter sequences differ only by 3 bp, T7 RNA polymerase only transcribes DNA cloned downstream of its promoter. ...
Here, we use 5′RACE-Seq to screen a randomized initially transcribed region of the T7 promoter for cross-talk with transcriptional activity. We reveal that sequences from position +4 to +8 downstream of the transcription start site affect T7 promoter activity over a 5-fold range, and...
CHARACTERIZATION OF T7 PROMOTER SEQUENCESdoi:DOI: 10.1016/B978-0-12-633360-2.50082-6Anthony D. CarterClaire E. MorrisWilliam T. McAllisterF.W. StudierMobilization & Reassembly of Genetic Information
promoters in which the AT-rich region from -17 to -22 is interrupted by several GC base pairs showed weak binding. Kinetic studies indicated that the presence of extended AT-rich sequence slows the dissociation rate constant of the polymerase-promoter complex and slightly stimulates the association...
Phage T3 DNA contains an exact copy of the 23 base-pair phage T7 RNA polymerase promoter sequence. J Mol Biol. 1981 Mar 25; 147 (1):41–53.Rosa M.D., Andrews N.C. Phage T3 DNA contains an exact copy of the 23 base-pair phage T7 RNA polymerase promoter sequence. J. Mol. ...
A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding anN-terminal HA tag in the T7 promoter-driven e... Rhim, "A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA ...
1b)7. The N-terminal domain plays a role in sequence specific promoter binding and in opening the duplex DNA. The region formed by the residues 93–101 bind the AT-rich −13 to −17 element of the promoter. An intercalating hairpin formed by residues 232–242 inserts between the ...
These results provided guidance for engineering novel RNAP–promoter pairs for synthetic biology or other applications. Our study also demonstrated the use of comprehensive genome mining in combination with sequence covariation analysis in the prediction and engineering of specific protein–DNA interactions....
Extended Upstream A-T Sequence Increases T7 Promoter Strengthdoi:10.1074/jbc.M508013200Guo Qing TangRajiv BandwarSmita PatelTang, G. Q., R. P. Bandwar, and S. S. Patel. 2005. Extended upstream A-T sequence increases T7 promoter strength. J Biol Chem 280:40707-13. :...