Genetransfer to plantsAgrobacterium binary vector systemA novel bacterial transposon, Tn 5-PV , that can used for mapping and analysis of the expression of genes transferred into plant cells, was constructed by insertion of a plant gene vector into Tn 5 without alteration of its transposition ...
Injection of a transposon expressing erythropoietin raised the haematocrit, indicating that the developed injection technique can elicit a biologic effect in vivo. Hydrodynamic renal pelvis injection enables transposon mediated-kidney specific gene transfer in adult mice. 展开 ...
To assess the activity of PB transposase to mediated gene transfer into the bovine genome, BFFs were transfected with 1 μg of pmGENIE-3 or pmhyGENIE-3 (self-inactivating hyperactive PB transposase-based plasmid). As control for random non-transpositional integration, BFFs were transfected in ...
mechanism of DNA transposition results in a single copy of the transgene per insertion locus, thus concatemer-induced gene silencing is unlikely to be an issue with transposition-mediated gene transfer. Indeed, Grabundzijaet al. found that transposon insertions delivered by theSB,Tol2andpiggyBac...
techniques such as lentiviral vector-mediated DNA transfer into early embryos, sperm-mediated transgenesis, embryo cloning by nuclear transfer and germline ... L Tesson,J Cozzi,S Ménoret,... - 《Transgenic Research》 被引量: 171发表: 2005年 New technology for an old favorite: lentiviral transg...
et al. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev. Cell 7, 133–144 (2004). Article CAS Google Scholar Davidson, A.E. et al. Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon. Dev. Biol. ...
PiggyBac transposon-mediated gene transfer in human cells. Transposons are mobile genetic elements that can be used to integrate transgenes into host cell genomes. ThepiggyBactransposon system has been used for tra... MH Wilson,CJ Coates,AL George - 《Molecular Therapy》 被引量: 718发表: 2007...
PB transposon-mediated gene transfer has been successfully performed in various organisms, both invertebrates and vertebrates. Studies in silkworm have benefited from this technology because the silk-gland bioreactor shows great potential for the production of vast quantities of valuable exogenous protein ...
TcBuster is a member of the hAT family of DNA transposons. Other members of the family include Sleeping Beauty and PiggBac. Discussed herein are various devices, systems and methods relating to synergistic approaches to enhance gene transfer into human hematopoietic and immune system cells using hAT...
We have developed an Agrobacterium-mediated gene transfer system in rice (Oryza sativa ssp. japonica ). Using this system, immature embryos, mature embryos and young panicles of rice variety Zhonghua No. 11 were used as explants to induce calli,respectively. The calli were infected and transforme...