Transposons are used as a gene therapy for hemophilia, and although not considered yet as a routine procedure, it is an ubiquitous tool in gene delivery and targeting in experimental systems. It discusses the growing use of it by researchers....
Gene therapy protocols require robust and long-term gene expression. For two decades, retrovirus family vectors have offered several attractive properties as stable gene-delivery vehicles. These vectors represent a technology with widespread use in basic biology and translational studies that require persis...
overview ends with a brief presentation of the use of TEs as biotechnology tools for insertional mutagenesis screening and gene therapy with DNA transposons... Benoit,Chenais - 《Current Cancer Drug Targets》 被引量: 14发表: 2015年 Risks of Insertional Mutagenesis by DNA Transposons in Cancer Gen...
Thus, DNA transposons are useful tools to analyze the regulatory genome, study embryonic development, identify genes and pathways implicated in disease or pathogenesis of pathogens, and even contribute to gene therapy. In this review, we will describe the nature of these elements and discuss recent...
While transposons are generally silenced in somatic tissues, many transposons escape epigenetic repression in epithelial cancers, become transcriptionally active and contribute to the regulation of human gene expression. We have developed a bioinformatic pipeline for the integrated analysis of transcription fac...
Hackett CS, Geurts AM, Wangensteen KJ, Balciunas D, Ekker SC, Hackett PB: Predicting transposon chromosomal insertion sites: implications for functional genomics and gene therapy. Genome Biology. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol...
The disclosure includes using a least one tniQ gene, and accordingly two or more different tniQ genes may be used. tniQ genes produce a TniQ protein that is an optional part of the present system. Including this gene in the construct will direct transposition event into the one specific co...
Applications of Sleeping Beauty transposons for nonviral gene therapy. IUBMB Life 2007;59:374-9.Liu H, Visner GA (2007) Applications of Sleeping beauty transposons for non- viral gene therapy. IUBMB Life 59, 374-379Liu H, Visner GA. Applications of sleeping beauty transposons for nonviral ...
Hyperactive piggyBac transposons for sustained and robust liver-targeted gene therapy. Mol Ther 2014;22:1614- 1624.Di Matteo, M. et al. Hyperactive PiggyBac Transposons for Sustained and Robust Liver-targeted Gene Therapy. Mol. Ther. 22, 1614-1624 (2014)....
These elements have been used for a variety of applications including germline mutagenesis, transgenesis, gene therapy, the production of induced pluripotent stem cells, and cancer genetics. The molecular characteristics are summarized for each TE currently used in the mouse. In addition, the current ...