Genetransfer to plantsAgrobacterium binary vector systemA novel bacterial transposon, Tn 5-PV , that can used for mapping and analysis of the expression of genes transferred into plant cells, was constructed by insertion of a plant gene vector into Tn 5 without alteration of its transposition ...
techniques such as lentiviral vector-mediated DNA transfer into early embryos, sperm-mediated transgenesis, embryo cloning by nuclear transfer and germline ... L Tesson,J Cozzi,S Ménoret,... - 《Transgenic Research》 被引量: 171发表: 2005年 New technology for an old favorite: lentiviral transg...
The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy... PR Score,L Belur,JL Frandsen,... - PubMed 被引量: 26发表: 2006年 加载更多来源...
Transposable elements can be viewed as natural DNA transfer vehicles that, similar to integrating viruses, are capable of efficient genomic insertion. The mobility of class II transposable elements (DNA transposons) can be controlled by conditionally providing the transposase component of the transposition...
Noun1.transposon- a segment of DNA that can become integrated at many different sites along a chromosome (especially a segment of bacterial DNA that can be translocated as a whole) jumping gene deoxyribonucleic acid,desoxyribonucleic acid,DNA- (biochemistry) a long linear polymer found in the nu...
et al. Transposon-mediated BAC transgenesis in zebrafish. Nat Protoc 6, 1998–2021 (2011). https://doi.org/10.1038/nprot.2011.416 Download citation Published01 December 2011 Issue DateDecember 2011 DOIhttps://doi.org/10.1038/nprot.2011.416 Subjects DNA transposable elements Genetic engineering This...
Transposon-mediated DNA delivery, although first limited to a small number of invertebrate species, has become increasingly more applicable to vertebrates, including mammalian cells. Preparations of non-viral plasmid-based transposon vectors are largely non-immunogenic, and have no hard constraints on ...
Agrobacterium-mediatedgenetransferDstranspositionOryzasalivaTransposonac/dsTwo kinds of T-DNA constructs, I-RS/dAc-I-RS and Hm(R)Ds, carrying a non-autono... Y Nakagawa,C Machida,Y Machida,... - 《Plant & Cell Physiology》 被引量: 167发表: 2000年 BIBAC and TAC clones containing potato ...
Transposon-mediated mutagenesis in somatic cells: identification of transposon-genomic DNA junctions. Methods Mol Biol, 2008, 435: 95-108.Largaespada DA, Collier LS (2008) Transposon-mediated mutagenesis in somatic cells: identification of transposon-genomic DNA junctions. Methods Mol Biol 435:95–...
Transposon-based transgenic methods, derived from naturally occurring DNA transposable elements, are nonviral gene delivery systems capable of efficient enzyme-mediated genomic insertion of DNA segments into the genome. During transposition, a single copy of the sequence of interest framed by inverted ...