The double transgene did not appear to cause embryonic lethality, as the mice were born at Mendellian ratios and were healthy for the first month of life. Genotyping primers are listed (Supplementary Table S12). Animal stud- ies were approved by the National Cancer Institute (NCI) ...
Thermo ScientificTM PhireTM Animal Tissue Direct PCR Kit is designed for DNA amplification directly from animal tissues. Here we present protocols for transgenic mice genotyping achieved directly from mouse ear tissues usi...
Results Motor performance and disease progression in R6/2 mice. Rigorous evaluation of motor coordina- tion to monitor disease progression and to precisely determine the advanced disease stage in our R6/2 line was performed using standardized procedures as previously described18. As expected, HD...
Acknowledgements We thank Ms Mei Li Lim, Shiau Chen H'ng and Ket Yin Goh for help in maintaining and genotyping the transgenic mice. This work was supported by a seed grant to BSW from the National University Health System (NUHSRO/2011/005/STB/B2B-01). ESC was supported by a graduate ...
Representative genotyping results from four mice that were produced by mating of heterozygous EμSRα-tTA (referred as tTA hereafter) and heterozygous Tet-o-wt Tax (referred as Tax hereafter) are shown in Fig. 1A. Lanes 1 and 2 show mice containing only the tTA or Tax transgenes ...
the use of this concept in aging research is inDrosophilausing transgenic strains that overexpress several differentenzymesand exhibit greater longevity and lower mortality than the wild type. Transgenic strains of bothC. elegansand mice have been produced but have not yet produced definitive results....
Types of Transgenic Mice Services Preparation of DNA for injection and development of a genotyping strategy The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ a special protocol to prepare top-quality DNA for our injection...
A total of 124 parvalbumin (PV)-Cre mice were generous gifts from Prof. You Wan (School of Medicine, Tsinghua University, Beijing, China). Mouse genotyping was performed following protocols described in the relevant literature [22,23,24]. P301L tau transgenic pR5 mice were generated on a ...
transgene PCR genotyping, and original generation of mouse and rabbit breeding and so on. Produced transgenic mice and rabbits can be used as disease models, or animal mammary gland bioreactors to produce bio-pharmaceutical enzymes, antibodies and other pharmaceutically important proteins. Normally, most...
Breeding and genotyping of a miR-5572 transgenic mouse model of sick sinus syndrome 来自 知网 喜欢 0 阅读量: 25 作者:J Zhang,LI Ying,J Fan 摘要: Objective This study aimed to explore the possibility of establishing a model of sick sinus syndrome by using miR-5572 transgenic mice. Methods ...