Schneider, MR, Wolf, E (2005) Genotyping of transgenic mice: old principles and recent developments. Anal Biochem 344: pp. 1-7Schneider, M. R. and Wolf, E. (2005). Genotyping of transgenic mice: Old principles and recent developments. Anal. Bio- chem. 344, 1-7....
An unbalanced increase in dietary omega-6 (n-6) polyunsaturated fatty acids (PUFA) and decrease in omega-3 (n-3) PUFA in the Western diet coincides with the global rise in chronic diseases. Whether n-6 and n-3 PUFA oppositely contribute to the developmen
A total of 124 parvalbumin (PV)-Cre mice were generous gifts from Prof. You Wan (School of Medicine, Tsinghua University, Beijing, China). Mouse genotyping was performed following protocols described in the relevant literature [22,23,24]. P301L tau transgenic pR5 mice were generated on a ...
Genotyping of the BAC Tg mice was performed by PCR analysis. PCR assays were designed using primers targeting the T7 and SP6 sequences in BAC vector pBACe3.6, and primers 59P20T7qR and 59P20SP6qR, which were specific to the 5′ and 3′ boundaries of mouse genomic DNA within the BAC...
The pronuclear injection (PI) is the simplest and widely used method to generate transgenic (Tg) mice. Unfortunately, PI-based Tg mice show uncertain transgene expression due to random transgene insertion in the genome, usually with multiple copies. Thus
Mice were housed at the Paracelsus Medical University Salzburg in groups under standard conditions at a temperature of 22 °C and a 12 h light/dark cycle with ad libitum access to standard food and water. Animal care, handling, genotyping and experiment were approved by local ethical committees...
1b). PCR genotyping using PTGER2 species-specific primers validated their mouse originality (Fig. 1c). The expression of the GFP tag was confirmed by Western blot analysis (Fig. 1d). The low level of GFP expression by MOC-L2 is consistent with its lower fluorescence and lower level of ...
Physical mapping of transgenic insertions by Fluorescence in situ Hybridization (FISH) in rodents is a well established and cost-effective technique which can be applied either independently for chromosomal assignment and zygosity status assessment [3, 4], or as a validation route in cases where high...
The GCaMP2 protein has already been applied in tissue preparations and in transgenic mice11–14, as well as in human pluripotent stem cells15, allowing calcium imaging without additional manipulation. However, a calcium sensor expressing rat model has not been available yet. Several methods are ...
Homozygosity was confirmed with Surveyor nuclease-based genotyping (Fig. 3i) and sequencing. However, homozygous knockout crickets died in the first-instar stage. To examine whether the ZFNs that were introduced caused off-target mutations, we performed a computational scan of a pre- liminary draft...