Thermo ScientificTM PhireTM Animal Tissue Direct PCR Kit is designed for DNA amplification directly from animal tissues. Here we present protocols for transgenic mice genotyping achieved directly from mouse ear tissues usi...
Further thymocyte fractionation demonstrated that DN1 and DN2, but not DN3 or DN4 fractions transmitted AML, and a marked expansion (100-fold) of Lineage- Sca1 + Kit + (LSK) cells in the thymus of the NP23-NHD13 mice. Taken together, these results show that the thymus of ...
www.nature.com/scientificreports OPEN Differential interaction of Apolipoprotein-E isoforms with insulin receptors modulates received: 01 April 2015 brain insulin signaling in mutantaccepted: 07August 2015 Published: 08 September 2015 human amyloid precursor protein transgenic mice Elizabeth S Chan1, ...
RNase Protection Assays and Quantitative Real Time Reverse Transcription-PCR—Total RNA was extracted using an RNeasy mini or micro kit (Qiagen) from Tax-expressing Jurkat cells (see supplemental data) or the skin of mice and then subjected to RNase protection assay according to the manufacturer'...
Generation of inducible COX2 transgenic mice A diagram of the COX2 transgene is shown inFig. 1A. In this construct, the cytomegalovirus early enhancer/chicken β-actin (CAG) promoter directs the expression of the human COX2 gene. However, the expression of human COX2 gene is blocked by a...
Targeting vulnerable microcircuits in the ventral hippocampus of male transgenic mice to rescue Alzheimer-like social memory loss Hui-Yang Lei, Gui-Lin Pi, Ting He, Rui Xiong, Jing-Ru Lv, Jia-Le Liu, Dong-Qin Wu, Meng-Zhu Li, Kun Shi, Shi-Hong Li, Na-Na Yu, Yang ...
Unlike mice, development of gene function via transgene delivery in mutant live offspring in live- stock have been slow to garner to attention due to low efficiency or severe mosaicism of microinjection and nuclear transfer18 at greater costs. Recently, SB transposon has been successfully applied ...
Carbery ID, Ji D, Harrington A, Brown V, Weinstein EJ, Liaw L, Cui X (2010) Targeted genome modification in mice using zinc-finger nucleases. Genetics 186:451–459 Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M, Voytas DF, Long CR, Whitelaw CBA, Fahrenkrug...
(deleted or mutated) in particular by homologous recombination. Preferably, said non-human transgenic mammals are homozygous for said inactivation; preferably, they are transgenic mice. Among the non-human transgenic mammals in which the endogenous locus of the immunoglobulin kappa light chain has been...
An unbalanced increase in dietary omega-6 (n-6) polyunsaturated fatty acids (PUFA) and decrease in omega-3 (n-3) PUFA in the Western diet coincides with the global rise in chronic diseases. Whether n-6 and n-3 PUFA oppositely contribute to the developmen